EC 188.8.131.52 - Amylo-α-1,6-glucosidase
IntEnz Enzyme Nomenclature
glycogen phosphorylase-limit dextrin α-1,6-glucohydrolase
- Hydrolysis of (1→6)-α-D-glucosidic branch linkages in glycogen phosphorylase limit dextrin
This enzyme hydrolyses an unsubstituted glucose unit linked by an α(1→6) bond to an α(1→4) glucose chain. The enzyme activity found in mammals and yeast is in a polypeptide chain containing two active centres. The other activity is similar to that of EC 184.108.40.206 (4-α-glucanotransferase), which acts on the glycogen phosphorylase limit dextrin chains to expose the single glucose residues, which the 6-α-glucosidase activity can then hydrolyse. Together, these two activities constitute the glycogen debranching system.
Links to other databases
Enzymes of glycogen debranching: Amylo-1,6-glucosidase (I) and oligo-1,4→1,4-glucanotransferase (II).Methods Enzymol. 8: 515-524 (1966).
Purification and properties of yeast amylo-1,6-glucosidase-oligo-1,4 leads to 1,4-glucantransferase.Biochemistry 9: 2347-2355 (1970). [PMID: 5424210]
Purification and properties of rabbit muscle amylo-1,6-glucosidase-oligo-1,4-1,4-transferase.Biochemistry 8: 1419-1428 (1969). [PMID: 5805288]
[EC 220.127.116.11 created 1965, modified 2000]