EC 3.2.1.179 - Gellan tetrasaccharide unsaturated glucuronosyl hydrolase

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IntEnz Enzyme Nomenclature
EC 3.2.1.179

Names

Accepted name:
gellan tetrasaccharide unsaturated glucuronosyl hydrolase
Other names:
UGL [ambiguous]
unsaturated glucuronyl hydrolase [ambiguous]
gellan tetrasaccharide unsaturated glucuronyl hydrolase
Systematic name:
β-D-4-deoxy-Δ4-GlcAp-(1→4)-β-D-Glcp-(1→4)-α-L-Rhap-(1→3)-D-Glcp β-D-4-deoxy-Δ4-GlcAp hydrolase

Reactions

Comments:

The enzyme releases 4-deoxy-4(5)-unsaturated D-glucuronic acid from oligosaccharides produced by polysaccharide lyases, e.g. the tetrasaccharide β-D-4-deoxy-Δ4-GlcAp-(1→4)-β-D-Glcp-(1→4)-α-L-Rhap-(1→3)-D-Glcp produced by EC 4.2.2.25, gellan lyase. The enzyme can also hydrolyse unsaturated chondroitin and hyaluronate disaccharides (β-D-4-deoxy-Δ4-GlcAp-(1→3)-D-GalNAc, β-D-4-deoxy-Δ4-GlcAp-(1→3)-D-GalNAc6S, β-D-4-deoxy-Δ4-GlcAp2S-(1→3)-D-GalNAc, β-D-4-deoxy-Δ4-GlcAp-(1→3)-D-GlcNAc), preferring the unsulfated disaccharides to the sulfated disaccharides.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Itoh, T., Akao, S., Hashimoto, W., Mikami, B., Murata, K.
    Crystal structure of unsaturated glucuronyl hydrolase, responsible for the degradation of glycosaminoglycan, from Bacillus sp. GL1 at 1.8 A resolution.
    J. Biol. Chem. 279: 31804-31812 (2004). [PMID: 15148314]
  2. Hashimoto, W., Kobayashi, E., Nankai, H., Sato, N., Miya, T., Kawai, S., Murata, K.
    Unsaturated glucuronyl hydrolase of Bacillus sp. GL1: novel enzyme prerequisite for metabolism of unsaturated oligosaccharides produced by polysaccharide lyases.
    Arch. Biochem. Biophys. 368: 367-374 (1999). [PMID: 10441389]
  3. Itoh, T., Hashimoto, W., Mikami, B., Murata, K.
    Substrate recognition by unsaturated glucuronyl hydrolase from Bacillus sp. GL1.
    Biochem. Biophys. Res. Commun. 344: 253-262 (2006). [PMID: 16630576]

[EC 3.2.1.179 created 2011, modified 2016]