EC 3.2.1.177 - α-D-xyloside xylohydrolase

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IntEnz Enzyme Nomenclature
EC 3.2.1.177

Names

Accepted name:
α-D-xyloside xylohydrolase
Other name:
α-xylosidase
Systematic name:
α-D-xyloside xylohydrolase

Reaction

Comments:

The enzyme catalyses hydrolysis of a terminal, unsubstituted xyloside at the extreme reducing end of a xylogluco-oligosaccharide. Representative α-xylosidases from glycoside hydrolase family 31 utilize a two-step (double-displacement) mechanism involving a covalent glycosyl-enzyme intermediate, and retain the anomeric configuration of the product.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0061634
UniProtKB/Swiss-Prot:

References

  1. Moracci, M., Cobucci Ponzano, B., Trincone, A., Fusco, S., De Rosa, M., van Der Oost, J., Sensen, C. W., Charlebois, R. L., Rossi, M.
    Identification and molecular characterization of the first α-xylosidase from an archaeon.
    J. Biol. Chem. 275: 22082-22089 (2000). [PMID: 10801892]
  2. Sampedro, J., Sieiro, C., Revilla, G., Gonzalez-Villa, T., Zarra, I.
    Cloning and expression pattern of a gene encoding an α-xylosidase active against xyloglucan oligosaccharides from Arabidopsis.
    Plant Physiol. 126: 910-920 (2001). [PMID: 11402218]
  3. Crombie, H. J., Chengappa, S., Jarman, C., Sidebottom, C., Reid, J. S.
    Molecular characterisation of a xyloglucan oligosaccharide-acting α-D-xylosidase from nasturtium (Tropaeolum majus L.) cotyledons that resembles plant 'apoplastic' α-D-glucosidases.
    Planta 214: 406-413 (2002). [PMID: 11859845]
  4. Lovering, A. L., Lee, S. S., Kim, Y. W., Withers, S. G., Strynadka, N. C.
    Mechanistic and structural analysis of a family 31 α-glycosidase and its glycosyl-enzyme intermediate.
    J. Biol. Chem. 280: 2105-2115 (2005). [PMID: 15501829]
  5. Iglesias, N., Abelenda, J. A., Rodino, M., Sampedro, J., Revilla, G., Zarra, I.
    Apoplastic glycosidases active against xyloglucan oligosaccharides of Arabidopsis thaliana.
    Plant Cell Physiol. 47: 55-63 (2006). [PMID: 16267099]
  6. Okuyama, M., Kaneko, A., Mori, H., Chiba, S., Kimura, A.
    Structural elements to convert Escherichia coli α-xylosidase (YicI) into α-glucosidase.
    FEBS Lett. 580: 2707-2711 (2006). [PMID: 16631751]
  7. Larsbrink, J., Izumi, A., Ibatullin, F. M., Nakhai, A., Gilbert, H. J., Davies, G. J., Brumer, H.
    Structural and enzymatic characterization of a glycoside hydrolase family 31 α-xylosidase from Cellvibrio japonicus involved in xyloglucan saccharification.
    Biochem. J. 436: 567-580 (2011). [PMID: 21426303]

[EC 3.2.1.177 created 2011]