EC 3.2.1.158 - α-agarase

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IntEnz Enzyme Nomenclature
EC 3.2.1.158

Names

Accepted name:
α-agarase
Other names:
agarase [ambiguous]
agarase A33
Systematic name:
agarose 3-glycanohydrolase

Reaction

Cofactor

Comments:

Requires Ca2+. The enzyme from Thalassomonas sp. can use agarose, agarohexaose and neoagarohexaose as substrate. The products of agarohexaose hydrolysis are dimers and tetramers, with agarotetraose being the predominant product, whereas hydrolysis of neoagarohexaose gives rise to two types of trimer. While the enzyme can also hydrolyse the highly sulfated agarose porphyran very efficiently, it cannot hydrolyse the related compounds κ-carrageenan (see EC 3.2.1.83) and ι-carrageenan (see EC 3.2.1.157) [2]. See also EC 3.2.1.81, β-agarase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033953
UniProtKB/Swiss-Prot:

References

  1. Potin, P., Richard, C., Rochas, C. and Kloareg, B.
    Purification and characterization of the α-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B.
    Eur. J. Biochem. 214: 599-607 (1993). [PMID: 8513809]
  2. Ohta, Y., Hatada, Y., Miyazaki, M., Nogi, Y., Ito, S. and Horikoshi, K.
    Purification and characterization of a novel α-agarase from a Thalassomonas sp.
    Curr. Microbiol. 50: 212-216 (2005). [PMID: 15902469]

[EC 3.2.1.158 created 2006]