EC 22.214.171.124 - α-agarase
IntEnz Enzyme Nomenclature
- Endohydrolysis of 1,3-α-L-galactosidic linkages in agarose, yielding agarotetraose as the major product
Requires Ca2+. The enzyme from Thalassomonas sp. can use agarose, agarohexaose and neoagarohexaose as substrate. The products of agarohexaose hydrolysis are dimers and tetramers, with agarotetraose being the predominant product, whereas hydrolysis of neoagarohexaose gives rise to two types of trimer. While the enzyme can also hydrolyse the highly sulfated agarose porphyran very efficiently, it cannot hydrolyse the related compounds κ-carrageenan (see EC 126.96.36.199) and ι-carrageenan (see EC 188.8.131.52) . See also EC 184.108.40.206, β-agarase.
Links to other databases
Purification and characterization of the α-agarase from Alteromonas agarlyticus (Cataldi) comb. nov., strain GJ1B.Eur. J. Biochem. 214: 599-607 (1993). [PMID: 8513809]
Purification and characterization of a novel α-agarase from a Thalassomonas sp.Curr. Microbiol. 50: 212-216 (2005). [PMID: 15902469]
[EC 220.127.116.11 created 2006]