EC 3.2.1.155 - Xyloglucan-specific exo-β-1,4-glucanase

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IntEnz Enzyme Nomenclature
EC 3.2.1.155

Names

Accepted name:
xyloglucan-specific exo-β-1,4-glucanase
Other name:
Cel74A
Systematic name:
[(1→6)-α-D-xylo]-(1→4)-β-D-glucan exo-glucohydrolase

Reaction

Comments:

The enzyme removes XXXG heptasaccharides, XXLG/XLXG octasaccharides and XLLG nonasaccharides from the end of tamarind seed xyloglucan polymers in a processive manner. Hydrolysis occurs at the unsubstituted D-glucopyranose residue in the main backbone. It is not known whether the cleavage takes place at the reducing or non-reducing end of the polymer. Very low activity with β-D-glucans. The enzyme from Chrysosporium lucknowense shifts to an endoglucanase mode when acting on linear substrates without bulky substituents on the polymeric backbone such as barley β-glucan. Formerly EC 3.2.1.160.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033950
UniProtKB/Swiss-Prot:

References

  1. Grishutin, S.G., Gusakov, A.V., Markov, A.V., Ustinov, B.B., Semenova, M.V. and Sinitsyn, A.P.
    Specific xyloglucanases as a new class of polysaccharide-degrading enzymes.
    Biochim. Biophys. Acta 1674: 268-281 (2004). [PMID: 15541296]

[EC 3.2.1.155 created 2005, withdrawn at public-review stage, modified and reinstated 2006, modified 2011]