EC 3.1.3.77 - Acireductone synthase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 3.1.3.77
Names
Accepted name:
acireductone synthase
Other
names:
E1
E-1 enolase-phosphatase
E-1 enolase-phosphatase
Systematic name:
5-(methylsulfanyl)-2,3-dioxopentyl-phosphate phosphohydrolase (isomerizing)
Reactions
- (1) 5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate
- (1a) 5-(methylsulfanyl)-2,3-dioxopentyl phosphate = 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate
- (1b) 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate + H2O = 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one + phosphate
Comments:
This bifunctional enzyme first enolizes the substrate to form the intermediate 2-hydroxy-5-(methylsulfanyl)-3-oxopent-1-enyl phosphate, which is then dephosphorylated to form the acireductone 1,2-dihydroxy-5-(methylsulfanyl)pent-1-en-3-one [2]. The acireductone represents a branch point in the methione-salvage pathway as it is used in the formation of formate, CO and 3-(methylsulfanyl)propanoate by EC 1.13.11.53 [acireductone dioxygenase (Ni2+-requiring)] and of formate and 4-(methylsulfanyl)-2-oxobutanoate either by a spontaneous reaction under aerobic conditions or by EC 1.13.11.54 {acireductone dioxygenase [iron(II)-requiring]} [1,2].
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
DIAGRAM
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
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MetaCyc
,
UniPathway
Gene Ontology:
GO:0043874
References
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Purification and characterization of an enzyme involved in oxidative carbon-carbon bond cleavage reactions in the methionine salvage pathway of Klebsiella pneumoniae.J. Biol. Chem. 268 : 24785-24791 (1993). [PMID: 8227039]
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The methionine salvage pathway in Klebsiella pneumoniae and rat liver. Identification and characterization of two novel dioxygenases.J. Biol. Chem. 270 : 3147-3153 (1995). [PMID: 7852397]
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Crystal structure of human E1 enzyme and its complex with a substrate analog reveals the mechanism of its phosphatase/enolase activity.J. Mol. Biol. 348 : 917-926 (2005). [PMID: 15843022]
[EC 3.1.3.77 created 2006]