EC 3.1.2.29 - Fluoroacetyl-CoA thioesterase

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IntEnz Enzyme Nomenclature
EC 3.1.2.29

Names

Accepted name:
fluoroacetyl-CoA thioesterase
Systematic name:
fluoroacetyl-CoA hydrolase

Reaction

Comments:

Fluoroacetate is extremely toxic. It reacts with CoA to form fluoroacetyl-CoA, which substitutes for acetyl CoA and reacts with EC 2.3.3.1 (citrate synthase) to produce fluorocitrate, a metabolite of which binds very tightly to EC 4.2.1.3 (aconitase) and halts the TCA cycle. This enzyme hydrolyses fluoroacetyl-CoA before it can react with citrate synthase, and thus confers fluoroacetate resistance on the organisms that produce it. It has been described in the poisonous plant Dichapetalum cymosum and the bacterium Streptomyces cattleya, both of which are fluoroacetate producers.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Meyer, J.J.M., Grobbelaar, N., Vleggaar, R. and Louw, A.I.
    Fluoroacetyl-coenzyme-A hydrolase-like activity in Dichapetalum cymosum.
    J. Plant Physiol. 139: 369-372 (1992).
  2. Huang, F., Haydock, S. F., Spiteller, D., Mironenko, T., Li, T. L., O'Hagan, D., Leadlay, P. F., Spencer, J. B.
    The gene cluster for fluorometabolite biosynthesis in Streptomyces cattleya: a thioesterase confers resistance to fluoroacetyl-coenzyme A.
    Chem. Biol. 13: 475-484 (2006). [PMID: 16720268]
  3. Dias, M. V., Huang, F., Chirgadze, D. Y., Tosin, M., Spiteller, D., Dry, E. F., Leadlay, P. F., Spencer, J. B., Blundell, T. L.
    Structural basis for the activity and substrate specificity of fluoroacetyl-CoA thioesterase FlK.
    J. Biol. Chem. 285: 22495-22504 (2010). [PMID: 20430898]

[EC 3.1.2.29 created 2011]