EC - Ribonuclease D

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
ribonuclease D
Other name:
RNase D
Systematic name:




Requires divalent cations for activity (Mg2+, Mn2+ or Co2+). Alteration of the 3'-terminal base has no effect on the rate of hydrolysis whereas modification of the 3'-terminal sugar has a major effect. tRNA terminating with a 3'-phosphate is completely inactive [3]. This enzyme can convert a tRNA precursor into a mature tRNA [2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033890
UniProtKB/Swiss-Prot: (20) [show] [UniProt]


  1. Ghosh, R.K. and Deutscher, M.P.
    Identification of an Escherichia coli nuclease acting on structurally altered transfer RNA molecules.
    J. Biol. Chem. 253 : 997-1000 (1978). [PMID: 342522]
  2. Cudny, H., Zaniewski, R. and Deutscher, M.P.
    Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease.
    J. Biol. Chem. 256 : 5627-5632 (1981). [PMID: 6263885]
  3. Cudny, H., Zaniewski, R. and Deutscher, M.P.
    Escherichia coli RNase D. Catalytic properties and substrate specificity.
    J. Biol. Chem. 256 : 5633-5637 (1981). [PMID: 6263886]
  4. Zhang, J. and Deutscher, M.P.
    Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D.
    J. Bacteriol. 170 : 522-527 (1988). [PMID: 2828310]

[EC created 2006]