EC 18.104.22.168 - Ribonuclease D
IntEnz Enzyme Nomenclature
- Exonucleolytic cleavage that removes extra residues from the 3'-terminus of tRNA to produce 5'-mononucleotides.
Requires divalent cations for activity (Mg2+, Mn2+ or Co2+). Alteration of the 3'-terminal base has no effect on the rate of hydrolysis whereas modification of the 3'-terminal sugar has a major effect. tRNA terminating with a 3'-phosphate is completely inactive . This enzyme can convert a tRNA precursor into a mature tRNA .
Links to other databases
Identification of an Escherichia coli nuclease acting on structurally altered transfer RNA molecules.J. Biol. Chem. 253: 997-1000 (1978). [PMID: 342522]
Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease.J. Biol. Chem. 256: 5627-5632 (1981). [PMID: 6263885]
Escherichia coli RNase D. Catalytic properties and substrate specificity.J. Biol. Chem. 256: 5633-5637 (1981). [PMID: 6263886]
Cloning, characterization, and effects of overexpression of the Escherichia coli rnd gene encoding RNase D.J. Bacteriol. 170: 522-527 (1988). [PMID: 2828310]
[EC 22.214.171.124 created 2006]