EC - Poly(3-hydroxyoctanoate) depolymerase

  IntEnz view ENZYME view

IntEnz Enzyme Nomenclature


Accepted name:
poly(3-hydroxyoctanoate) depolymerase
Other names:
PHO depolymerase
poly(3HO) depolymerase
poly(HA) depolymerase
poly(HAMCL) depolymerase
poly[(R)-hydroxyalkanoic acid] depolymerase
poly[(R)-3-hydroxyoctanoate] hydrolase
Systematic name:
poly{oxycarbonyl[(R)-2-pentylethylene]} hydrolase



The main product after prolonged incubation is the dimer [3]. Besides hydrolysing polymers of 3-hydroxyoctanoic acid, the enzyme also hydrolyses other polymers derived from medium-chain-length (C6-C12) hydroxyalkanoic acids and copolymers of mixtures of these. It also hydrolyses p-nitrophenyl esters of fatty acids. Polymers of short-chain-length hydroxyalkanoic acids such as poly[(R)-3-hydroxybutanoic acid] and poly[(R)-3-hydroxypentanoic acid] are not hydrolysed.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050527


  1. Jendrossek, D.
    Microbial degradation of polyesters.
    Adv. Biochem. Eng./Biotechnol. 71: 293-325 (2001). [PMID: 11217416]
  2. García, B., Olivera, E.R., Miñambres, B., Fernández-Valverde, Cañedo, L.M., Prieto, M.A., García, J.L., Martínez, M. and Luengo, J.M.
    Novel biodegradable aromatic plastics from a bacterial source. Genetic and biochemical studies on a route of the phenylacetyl-CoA catabolon.
    J. Biol. Chem. 274: 29228-29241 (1999). [PMID: 10506180]
  3. Schirmer, A., Jendrossek, D. and Schlegel, H.G.
    Degradation of poly(3-hydroxyoctanoic acid) [P(3HO)] by bacteria: purification and properties of a P(3HO) depolymerase from Pseudomonas fluorescens GK13.
    Appl. Environ. Microbiol. 59: 1220-1227 (1993). [PMID: 8476295]

[EC created 2001, modified 2005]