EC 2.9.1.2 - O-phospho-L-seryl-tRNASec:L-selenocysteinyl-tRNA synthase

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IntEnz Enzyme Nomenclature
EC 2.9.1.2

Names

Accepted name:
O-phospho-L-seryl-tRNASec:L-selenocysteinyl-tRNA synthase
Other names:
MMPSepSecS
SepSecS
SLA/LP
O-phosphoseryl-tRNA:selenocysteinyl-tRNA synthase
O-phospho-L-seryl-tRNA:L-selenocysteinyl-tRNA synthase
O-phosphoseryl-tRNASec selenium transferase
Systematic name:
selenophosphate:O-phospho-L-seryl-tRNASec selenium transferase

Reaction

Cofactor

Comments:

A pyridoxal-phosphate protein [4]. In archaea and eukarya selenocysteine formation is achieved by a two-step process: EC 2.7.1.164 (O-phosphoseryl-tRNASec kinase) phosphorylates the endogenous L-seryl-tRNASec to O-phospho-L-seryl-tRNASec, and then this misacylated amino acid-tRNA species is converted to L-selenocysteinyl-tRNASec by Sep-tRNA:Sec-tRNA synthase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (11) [show] [UniProt]

References

  1. Palioura, S., Sherrer, R. L., Steitz, T. A., Soll, D., Simonovic, M.
    The human SepSecS-tRNASec complex reveals the mechanism of selenocysteine formation.
    Science 325: 321-325 (2009). [PMID: 19608919]
  2. Araiso, Y., Palioura, S., Ishitani, R., Sherrer, R. L., O'Donoghue, P., Yuan, J., Oshikane, H., Domae, N., Defranco, J., Soll, D., Nureki, O.
    Structural insights into RNA-dependent eukaryal and archaeal selenocysteine formation.
    Nucleic Acids Res. 36: 1187-1199 (2008). [PMID: 18158303]
  3. Aeby, E., Palioura, S., Pusnik, M., Marazzi, J., Lieberman, A., Ullu, E., Soll, D., Schneider, A.
    The canonical pathway for selenocysteine insertion is dispensable in Trypanosomes.
    Proc. Natl. Acad. Sci. USA 106: 5088-5092 (2009). [PMID: 19279205]
  4. Yuan, J., Palioura, S., Salazar, J. C., Su, D., O'Donoghue, P., Hohn, M. J., Cardoso, A. M., Whitman, W. B., Soll, D.
    RNA-dependent conversion of phosphoserine forms selenocysteine in eukaryotes and archaea.
    Proc. Natl. Acad. Sci. USA 103: 18923-18927 (2006). [PMID: 17142313]

[EC 2.9.1.2 created 2009]