EC 2.8.2.30 - [heparan sulfate]-glucosamine 3-sulfotransferase 3

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IntEnz Enzyme Nomenclature
EC 2.8.2.30

Names

Accepted name:
[heparan sulfate]-glucosamine 3-sulfotransferase 3
Other names:
glucosaminyl 3-O-sulfotransferase 3a, 3b
heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A
isoform/isozyme 3a and 3b (3-OST-3A, 3-OST-3B, HS3ST3A, HS3ST3B)
Systematic name:
3'-phosphoadenylyl-sulfate:[heparan sulfate]-glucosamine 3-sulfotransferase

Reaction

Comments:

Two major substrates contain the tetrasaccharides: →undetermined 2-sulfo-uronic acid→GlcN2S→ IdoA2S→GlcN*→ and →undetermined 2-sulfo-uronic acid→GlcN2S→ IdoA2S→GlcN6S*→ (symbols as in 2-Carb-38) with modification of the N-unsubstituted glucosamine residue (shown with an asterisk) [1,4]. Modification of selected sequences containing N-sulfo-glucosamine residues cannot yet be excluded. The 3-O-sulfated heparan sulfate can be utilized by Herpes simplex virus type 1 as an entry receptor to infect the target cells [2]. There are two isozymes, known as 3-OST-3A and 3-OST-3B, which have identical catalytic domains but are encoded by different mammalian genes [3]. The specificity of this enzyme differs from that of the other [heparan sulfate]-glucosamine 3-sulfotransferases. It is inefficient at modifying precursors of the antithrombin binding site (in contrast to EC 2.8.2.23 ([heparan sulfate]-glucosamine 3-sulfotransferase 1)] and it does not modify glucosamine preceded by GlcA2S [unlike EC 2.8.2.29 ([heparan sulfate]-glucosamine 3-sulfotransferase 2)].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033872
UniProtKB/Swiss-Prot:

References

  1. Heparan sulfate D-glucosaminyl 3-O-sulfotransferase 3A sulfates N-unsubstituted glucosamine.
    J. Biol. Chem. 274: 38155-38162 (1999). [PMID: 10608887]
  2. Shukla, D., Liu, J., Blaiklock, P., Shworak, N.W., Bai, X., Esko, J.D., Cohen, G.H., Eisenberg, R.J., Rosenberg, R.D. and Spear, P.G.
    A novel role for 3-O-sulfated heparan sulfate in Herpes simplex virus 1 entry.
    Cell 99: 13-22 (1999). [PMID: 10520990]
  3. Shworak, N.W., Liu, J., Petros, L.M., Copeland, N.G., Jenkins N.A. and Rosenberg, R.D.
    Diversity of the extensive heparan sulfate D-glucosaminyl 3-O-sulfotransferase (3-OST) multigene family.
    J. Biol. Chem. 274: 5170-5184 (1999). [PMID: 9988767]
  4. Liu, J., Shworak, N.W., Sina, P., Schwartz, J.J., Zhang, L., Fritze, L.M.S. and Rosenberg, R.D.
    Expression of heparan sulfate D-glucosaminyl 3-O-sulfotransferase isoforms reveals novel substrate specificities.
    J. Biol. Chem. 274: 5185-5192 (1999). [PMID: 9988768]

[EC 2.8.2.30 created 2001]