EC - 3-mercaptopyruvate sulfurtransferase

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IntEnz Enzyme Nomenclature


Accepted name:
3-mercaptopyruvate sulfurtransferase
Other names:
β-mercaptopyruvate sulfurtransferase
TUM1 (gene name)
MPST (gene name)
3-mercaptopyruvate:cyanide sulfurtransferase
Systematic name:
3-mercaptopyruvate:sulfide sulfurtransferase




The enzyme catalyses a transsulfuration reaction from 3-mercaptopyruvate to an internal cysteine residue. In the presence of a dithiol such as reduced thioredoxin or dihydrolipoate, the sulfanyl sulfur is released as hydrogen sulfide. The enzyme participates in a sulfur relay process that leads to the 2-thiolation of some tRNAs and to protein urmylation by transferring sulfur between the NFS1 cysteine desulfurase (EC and the MOCS3 sulfurtransferase (EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00322
Structural data: CSA , EC2PDB
Gene Ontology: GO:0016784
CAS Registry Number: 9026-05-5


  1. Fiedler, H. and Wood, J.L.
    Specificity studies on the β-mercaptopyruvate-cyanide transsulfuration system.
    J. Biol. Chem. 222: 387-397 (1956). [PMID: 13367011]
  2. Hylin, J.W. and Wood, J.L.
    Enzymatic formation of polysulfides from mercaptopyruvate.
    J. Biol. Chem. 234: 2141-2144 (1959). [PMID: 13673028]
  3. Sörbo, B.H.
    Enzymic transfer of sulfur from mercaptopyruvate to sulfite or sulfinates.
    Biochem. Biophys. Acta 24: 324-329 (1957). [PMID: 13436433]
  4. Vachek, H. and Wood, J.L.
    Purification and properties of mercaptopyruvate sulfur transferase of Escherichia coli.
    Biochim. Biophys. Acta 258: 133-146 (1972). [PMID: 4550801]
  5. van den Hamer, C.J.A., Morell, A.G. and Scheinberg, H.I.
    A study of the copper content of β-mercaptopyruvate trans-sulfurase.
    J. Biol. Chem. 242: 2514-2516 (1967). [PMID: 6026243]
  6. Nagahara, N., Katayama, A.
    Post-translational regulation of mercaptopyruvate sulfurtransferase via a low redox potential cysteine-sulfenate in the maintenance of redox homeostasis.
    J. Biol. Chem. 280: 34569-34576 (2005). [PMID: 16107337]
  7. Shibuya, N., Tanaka, M., Yoshida, M., Ogasawara, Y., Togawa, T., Ishii, K., Kimura, H.
    3-Mercaptopyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain.
    Antioxid. Redox Signal. 11: 703-714 (2009). [PMID: 18855522]
  8. Mikami, Y., Shibuya, N., Kimura, Y., Nagahara, N., Ogasawara, Y., Kimura, H.
    Thioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide.
    Biochem. J. 439: 479-485 (2011). [PMID: 21732914]

[EC created 1961, modified 2018]