EC 2.8.1.2 - 3-mercaptopyruvate sulfurtransferase

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IntEnz Enzyme Nomenclature
EC 2.8.1.2

Names

Accepted name:
3-mercaptopyruvate sulfurtransferase
Other names:
β-mercaptopyruvate sulfurtransferase
TUM1 (gene name)
MPST (gene name)
3-mercaptopyruvate:cyanide sulfurtransferase
Systematic name:
3-mercaptopyruvate:sulfide sulfurtransferase

Reaction

Cofactor

Comments:

The enzyme catalyses a transsulfuration reaction from 3-mercaptopyruvate to an internal cysteine residue. In the presence of a dithiol such as reduced thioredoxin or dihydrolipoate, the sulfanyl sulfur is released as hydrogen sulfide. The enzyme participates in a sulfur relay process that leads to the 2-thiolation of some tRNAs and to protein urmylation by transferring sulfur between the NFS1 cysteine desulfurase (EC 2.8.1.7) and the MOCS3 sulfurtransferase (EC 2.8.1.11).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00322
Structural data: CSA , EC2PDB
Gene Ontology: GO:0016784
CAS Registry Number: 9026-05-5
UniProtKB/Swiss-Prot:

References

  1. Fiedler, H. and Wood, J.L.
    Specificity studies on the β-mercaptopyruvate-cyanide transsulfuration system.
    J. Biol. Chem. 222: 387-397 (1956). [PMID: 13367011]
  2. Hylin, J.W. and Wood, J.L.
    Enzymatic formation of polysulfides from mercaptopyruvate.
    J. Biol. Chem. 234: 2141-2144 (1959). [PMID: 13673028]
  3. Sörbo, B.H.
    Enzymic transfer of sulfur from mercaptopyruvate to sulfite or sulfinates.
    Biochem. Biophys. Acta 24: 324-329 (1957). [PMID: 13436433]
  4. Vachek, H. and Wood, J.L.
    Purification and properties of mercaptopyruvate sulfur transferase of Escherichia coli.
    Biochim. Biophys. Acta 258: 133-146 (1972). [PMID: 4550801]
  5. van den Hamer, C.J.A., Morell, A.G. and Scheinberg, H.I.
    A study of the copper content of β-mercaptopyruvate trans-sulfurase.
    J. Biol. Chem. 242: 2514-2516 (1967). [PMID: 6026243]
  6. Nagahara, N., Katayama, A.
    Post-translational regulation of mercaptopyruvate sulfurtransferase via a low redox potential cysteine-sulfenate in the maintenance of redox homeostasis.
    J. Biol. Chem. 280: 34569-34576 (2005). [PMID: 16107337]
  7. Shibuya, N., Tanaka, M., Yoshida, M., Ogasawara, Y., Togawa, T., Ishii, K., Kimura, H.
    3-Mercaptopyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain.
    Antioxid. Redox Signal. 11: 703-714 (2009). [PMID: 18855522]
  8. Mikami, Y., Shibuya, N., Kimura, Y., Nagahara, N., Ogasawara, Y., Kimura, H.
    Thioredoxin and dihydrolipoic acid are required for 3-mercaptopyruvate sulfurtransferase to produce hydrogen sulfide.
    Biochem. J. 439: 479-485 (2011). [PMID: 21732914]

[EC 2.8.1.2 created 1961, modified 2018]