EC 2.7.8.7 - Holo-[acyl-carrier-protein] synthase

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IntEnz Enzyme Nomenclature
EC 2.7.8.7

Names

Accepted name:
holo-[acyl-carrier-protein] synthase
Other names:
acyl carrier protein holoprotein (holo-ACP) synthetase
acyl carrier protein synthetase
coenzyme A:fatty acid synthetase apoenzyme 4'-phosphopantetheine transferase
holo-ACP synthase
holo-ACP synthetase
holosynthase
PPTase
AcpS
ACPS
acyl carrier protein synthase
P-pant transferase
CoA:apo-[acyl-carrier-protein] pantetheinephosphotransferase
L-aminoadipate-semialdehyde dehydrogenase-phosphopantetheinyl transferase
4'-phosphopantetheinyl transferase
αaminoadipic semialdehyde dehydrogenase-phosphopantetheinyl transferase
Systematic name:
CoA-[4'-phosphopantetheine]:apo-[acyl-carrier-protein] 4'-pantetheinephosphotransferase

Reaction

Cofactor

Comments:

Requires Mg2+. All polyketide synthases, fatty-acid synthases and non-ribosomal peptide synthases require post-translational modification of their constituent acyl-carrier-protein (ACP) domains to become catalytically active. The inactive apo-proteins are converted into their active holo-forms by transfer of the 4'-phosphopantetheinyl moiety of CoA to the sidechain hydroxy group of a conserved serine residue in each ACP domain [3]. The enzyme from human can activate both the ACP domain of the human cytosolic multifunctional fatty acid synthase system (EC 2.3.1.85) and that associated with human mitochondria as well as peptidyl-carrier and acyl-carrier-proteins from prokaryotes [6]. Removal of the 4-phosphopantetheinyl moiety from holo-ACP is carried out by EC 3.1.4.14, [acyl-carrier-protein] phosphodiesterase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0008897
CAS Registry Number: 37278-30-1
UniProtKB/Swiss-Prot: (595) [show] [UniProt]

References

  1. Elovson, J. and Vagelos, P.R.
    Acyl carrier protein. X. Acyl carrier protein synthetase.
    J. Biol. Chem. 243 : 3603-3611 (1968). [PMID: 4872726]
  2. Prescott, D.J. and Vagelos, P.R.
    Acyl carrier protein.
    Adv. Enzymol. Relat. Areas Mol. Biol. 36 : 269-311 (1972). [PMID: 4561013]
  3. Lambalot, R.H., Gehring, A.M., Flugel, R.S., Zuber, P., LaCelle, M., Marahiel, M.A., Reid, R., Khosla, C. and Walsh, C.T.
    A new enzyme superfamily - the phosphopantetheinyl transferases.
    Chem. Biol. 3 : 923-936 (1996). [PMID: 8939709]
  4. Walsh, C.T., Gehring, A.M., Weinreb, P.H., Quadri, L.E.N. and Flugel, R.S.
    Post-translational modification of polyketide and nonribosomal peptide synthases.
    Curr. Opin. Chem. Biol. 1 : 309-315 (1997). [PMID: 9667867]
  5. Mootz, H.D., Finking, R. and Marahiel, M.A.
    4'-phosphopantetheine transfer in primary and secondary metabolism of Bacillus subtilis.
    J. Biol. Chem. 276 : 37289-37298 (2001). [PMID: 11489886]
  6. Joshi, A.K., Zhang, L., Rangan, V.S. and Smith, S.
    Cloning, expression, and characterization of a human 4'-phosphopantetheinyl transferase with broad substrate specificity.
    J. Biol. Chem. 278 : 33142-33149 (2003). [PMID: 12815048]

[EC 2.7.8.7 created 1972, modified 2006]