IntEnz

EC 2.7.7.60 - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

IntEnz view ENZYME view

XML

IntEnz Enzyme Nomenclature
EC 2.7.7.60

Names

Accepted name:

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Other names:

MEP cytidylyltransferase
4-diphosphocytidyl-2-C-methyl-D-erythritol synthase

Systematic name:

CTP:2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Reaction

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

Cofactor

Mn²⁺ Mg²⁺

Comments:

The enzyme from Escherichia coli requires Mg2+ or Mn2+. ATP or UTP can replace CTP, but both are less effective. GTP and TTP are not substrates. Forms part of an alternative nonmevalonate pathway for terpenoid biosynthesis.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway

Structural data: CSA , EC2PDB

Gene Ontology: GO:0050518

UniProtKB/Swiss-Prot: (455) [show] [UniProt]

References

Kuzuyama, T., Takagi, M., Kaneda, K., Dairi, T. and Seto, H.

Formation of 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol from 2-C-methyl-D-erythritol 4-phosphate by 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase, a new enzyme in the nonmevalonate pathway.

Tetrahedron Lett. 41 : 703-706 (2000).
Rohdich, F., Wungsintaweekul, J., Fellermeier, M., Sagner, S., Herz, S., Kis, K., Eisenreich, W., Bacher, A. and Zenk, M.H.

Cytidine 5'-triphosphate-dependent biosynthesis of isoprenoids: YgbP protein of Escherichia coli catalyzes the formation of 4-diphosphocytidyl-2-C-methylerythritol.

Proc. Natl. Acad. Sci. USA 96 : 11758-11763 (1999). [PMID: 10518523]

[EC 2.7.7.60 created 2001]

EC 2 - Transferases

EC 2.7 - Transferring phosphorus-containing groups