IntEnz

EC 2.7.7.2 - FAD synthetase

IntEnz Enzyme Nomenclature
EC 2.7.7.2

Names

Reaction

• 17237 [IUBMB]
  ATP

  ATP

  Name origin: UniProt - CHECKED (C)

  CHEBI:30616

  Formula: C10H12N5O13P3
  Charge: -4

  ChEBI compound status: CHECKED (C)

  

  +

  FMN

  FMN

  Name origin: UniProt - CHECKED (C)

  CHEBI:58210

  Formula: C17H18N4O9P
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  =

  diphosphate

  diphosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:33019

  Formula: HO7P2
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

  +

  FAD

  FAD

  Name origin: UniProt - CHECKED (C)

  CHEBI:57692

  Formula: C27H30N9O15P2
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

Cofactor

• Mg²⁺

Comments:

Requires Mg²⁺ and is highly specific for ATP as phosphate donor [5]. The cofactors FMN and FAD participate in numerous processes in all organisms, including mitochondrial electron transport, photosynthesis, fatty-acid oxidation, and metabolism of vitamin B₆, vitamin B₁₂ and folates [3]. While monofunctional FAD synthetase is found in eukaryotes and in some prokaryotes, most prokaryotes have a bifunctional enzyme that exhibits both this activity and that of EC 2.7.1.26, riboflavin kinase [3,5].

Links to other databases

References

1. Giri, K.V., Rao, N.A., Cama, H.R. and Kumar, S.A.
   Studies on flavinadenine dinucleotide-synthesizing enzyme in plants.
   Biochem. J. 75: 381-386 (1960). [PMID: 13828163]
2. Schrecker, A.W. and Kornberg, A.
   Reversible enzymatic synthesis of flavin-adenine dinucleotide.
   J. Biol. Chem. 182: 795-803 (1950).
3. Sandoval, F.J. and Roje, S.
   An FMN hydrolase is fused to a riboflavin kinase homolog in plants.
   J. Biol. Chem. 280: 38337-38345 (2005). [PMID: 16183635]
4. Oka, M. and McCormick, D.B.
   Complete purification and general characterization of FAD synthetase from rat liver.
   J. Biol. Chem. 262: 7418-7422 (1987). [PMID: 3034893]
5. Brizio, C., Galluccio, M., Wait, R., Torchetti, E. M., Bafunno, V., Accardi, R., Gianazza, E., Indiveri, C., Barile, M.
   Over-expression in Escherichia coli and characterization of two recombinant isoforms of human FAD synthetase.
   Biochem. Biophys. Res. Commun. 344: 1008-1016 (2006). [PMID: 16643857]

[EC 2.7.7.2 created 1961, modified 2007]