EC - Protein-Nπ-phosphohistidine—D-mannose phosphotransferase

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IntEnz Enzyme Nomenclature


Accepted name:
protein-Nπ-phosphohistidine—D-mannose phosphotransferase
Other names:
manXYZ (gene names)
mannose PTS permease
Enzyme IIMan
Systematic name:
protein-Nπ-phospho-L-histidine:D-mannose Nπ-phosphotransferase



This enzyme is a component (known as enzyme II) of a phosphoenolpyruvate (PEP)-dependent, sugar transporting phosphotransferase system (PTS). The system, which is found only in prokaryotes, simultaneously transports its substrate from the periplasm or extracellular space into the cytoplasm and phosphorylates it. The phosphate donor, which is shared among the different systems, is a phospho-carrier protein of low molecular mass that has been phosphorylated by EC (phosphoenolpyruvate—protein phosphotransferase). Enzyme II, on the other hand, is specific for a particular substrate, although in some cases alternative substrates can be transported with lower efficiency. The reaction involves a successive transfer of the phosphate group to several amino acids within the enzyme before the final transfer to the substrate.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00795
Structural data: CSA , EC2PDB


  1. Erni, B., Zanolari, B.
    The mannose-permease of the bacterial phosphotransferase system. Gene cloning and purification of the enzyme IIMan/IIIMan complex of Escherichia coli.
    J. Biol. Chem. 260 : 15495-15503 (1985). [PMID: 2999119]
  2. Williams, N., Fox, D. K., Shea, C., Roseman, S.
    Pel, the protein that permits lambda DNA penetration of Escherichia coli, is encoded by a gene in ptsM and is required for mannose utilization by the phosphotransferase system.
    Proc. Natl. Acad. Sci. U.S.A. 83 : 8934-8938 (1986). [PMID: 2947241]
  3. Erni, B., Zanolari, B., Kocher, H. P.
    The mannose permease of Escherichia coli consists of three different proteins. Amino acid sequence and function in sugar transport, sugar phosphorylation, and penetration of phage lambda DNA.
    J. Biol. Chem. 262 : 5238-5247 (1987). [PMID: 2951378]
  4. Stolz, B., Huber, M., Markovi?-Housley, Z., Erni, B.
    The mannose transporter of Escherichia coli. Structure and function of the IIABMan subunit.
    J. Biol. Chem. 268 : 27094-27099 (1993). [PMID: 8262947]
  5. Rhiel, E., Flukiger, K., Wehrli, C., Erni, B.
    The mannose transporter of Escherichia coli K12: oligomeric structure, and function of two conserved cysteines.
    Biol. Chem. Hoppe-Seyler 375 : 551-559 (1994). [PMID: 7811395]
  6. Huber, F., Erni, B.
    Membrane topology of the mannose transporter of Escherichia coli K12.
    Eur. J. Biochem. 239 : 810-817 (1996). [PMID: 8774730]

[EC created 1972 as EC, part transferred 2016 to EC]