EC - 6-phosphofructokinase

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IntEnz Enzyme Nomenclature


Accepted name:
Other names:
6-phosphofructose 1-kinase
D-fructose-6-phosphate 1-phosphotransferase
ATP-dependent phosphofructokinase
fructose 6-phosphate kinase
fructose 6-phosphokinase
nucleotide triphosphate-dependent phosphofructokinase
phosphofructokinase (phosphorylating)
phosphofructokinase I
Systematic name:
ATP:β-D-fructose-6-phosphate 1-phosphotransferase



The enzyme from rabbit muscle displays absolute stereoselectivity for the β-anomer of D-fructofuranose 6-phosphate [9-11]. D-Tagatose 6-phosphate and sedoheptulose 7-phosphate can act as acceptors. UTP, CTP and ITP can act as donors. Not identical with EC 6-phosphofructo-2-kinase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00336 , PROSITE:PDOC00504
Structural data: CSA , EC2PDB
Gene Ontology: GO:0003872
CAS Registry Number: 9001-80-3
UniProtKB/Swiss-Prot: (346) [show] [UniProt]


  1. Axelrod, B., Saltman, P., Bandurski, R.S. and Baker, R.S.
    Hexokinase in higher plants.
    J. Biol. Chem. 197 : 89-96 (1952). [PMID: 12981037]
  2. Ling, K.H., Pastkau, V., Marcus, F. and Lardy, H.A.
    Phosphofructokinase. I. Skeletal muscle.
    Methods Enzymol. 9 : 425-429 (1966).
  3. Mansour, T.E.
    Phosphofructokinase. II. Heart muscle.
    Methods Enzymol. 9 : 430-436 (1966).
  4. Odeide, R., Guilloton, M., Dupuis, B., Ravon, D. and Rosenberg, A.J.
    Study of an enzyme allosteric to 2 substrates: phosphofructokinase of rat muscle. I. Preparation and crystallization of the enzyme.
    Bull. Soc. Chim. Biol. 50 : 2023-2033 (1968). [PMID: 4237772]
  5. Parmeggiano, A., Luft, J.H., Love, D.S. and Krebs, E.G.
    Crystallization and properties of rabbit skeletal muscle phosphofructokinase.
    J. Biol. Chem. 241 : 4625-4637 (1966). [PMID: 4224472]
  6. Racker, E.
    Spectrophotometric measurement of hexokinase and phosphohexokinase activity.
    J. Biol. Chem. 167 : 843-854 (1947). [PMID: 20287918]
  7. Sols, A. and Salas, M.L.
    Phosphofructokinase. III. Yeast.
    Methods Enzymol. 9 : 436-442 (1966).
  8. Uyeda, K. and Kurooka, S.
    Crystallization and properties of phosphofructokinase from Clostridium pasteurianum.
    J. Biol. Chem. 245 : 3315-3324 (1970). [PMID: 4248230]
  9. Fishbein, R., Benkovic, P. A., Schray, K. J., Siewers, I. J., Steffens, J. J., Benkovic, S. J.
    Anomeric specificity of phosphofructokinase from rabbit muscle.
    J Biol Chem 249 : 6047-6051 (1974). [PMID: 4278654]
  10. Wurster, B., Hess, B.
    Anomeric specificity of fructose-6-phosphate kinase (EC from rabbit muscle.
    FEBS Lett 38 : 257-260 (1974). [PMID: 4277364]
  11. Koerner, T. A., Younathan, E. S., Ashour, A. L., Voll, R. J.
    The fructose 6-phosphate site of phosphofructokinase. I. Tautomeric and anomeric specificity.
    J Biol Chem 249 : 5749-5754 (1974). [PMID: 4278316]

[EC created 1961]