EC 2.5.1.98 - Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase

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IntEnz Enzyme Nomenclature
EC 2.5.1.98

Names

Accepted name:
Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase
Other names:
PssM
phosphoenolpyruvate:[D-GlcA-β-(1→4)-2-O-Ac-D-GlcA-β-(1→4)-D-Glc-β-(1→4)-[3-O-CH3-CH2CH(OH)C(O)-D-Gal-β-(1→4)-D-Glc-β-(1→4)-D-Glc-β-(1→4)-D-Glc-β-(1→6)]-2(or3)-O-Ac-D-Glc-α-(1→6)]n 4,6-O-(1-carboxyethan-1,1-diyl)transferase
Systematic name:
phosphoenolpyruvate:[β-D-GlcA-β-(1→4)-2-O-Ac-β-D-GlcA-(1→4)-β-D-Glc-(1→4)-[3-O-(CH3-CH2CH(OH)C(O))-4,6-CH3(COO)C-β-D-Gal-(1→4)-β-D-Glc-(1→4)-β-D-Glc-(1→4)-β-D-Glc-(1→6)]-2(or3)-O-Ac-α-D-Glc-(1→6)]n 4,6-O-(1-carboxyethan-1,1-diyl)transferase

Reaction

Comments:

The enzyme is responsible for pyruvylation of subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium leguminosarum (bv. viciae strain VF39) which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Ivashina, T. V., Fedorova, E. E., Ashina, N. P., Kalinchuk, N. A., Druzhinina, T. N., Shashkov, A. S., Shibaev, V. N., Ksenzenko, V. N.
    Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis.
    J. Appl. Microbiol. 109 : 731-742 (2010). [PMID: 20233262]

[EC 2.5.1.98 created 2012, modified 2018]