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EC 2.5.1.98 - Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase

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IntEnz Enzyme Nomenclature
EC 2.5.1.98

Names

Accepted name:

Rhizobium leguminosarum exopolysaccharide glucosyl ketal-pyruvate-transferase

Other names:

PssM
phosphoenolpyruvate:[D-GlcA-β-(1→4)-2-O-Ac-D-GlcA-β-(1→4)-D-Glc-β-(1→4)-[3-O-CH₃-CH₂CH(OH)C(O)-D-Gal-β-(1→4)-D-Glc-β-(1→4)-D-Glc-β-(1→4)-D-Glc-β-(1→6)]-2(or3)-O-Ac-D-Glc-α-(1→6)]_n 4,6-O-(1-carboxyethan-1,1-diyl)transferase

Systematic name:

phosphoenolpyruvate:[β-D-GlcA-β-(1→4)-2-O-Ac-β-D-GlcA-(1→4)-β-D-Glc-(1→4)-[3-O-(CH₃-CH₂CH(OH)C(O))-4,6-CH₃(COO⁻)C-β-D-Gal-(1→4)-β-D-Glc-(1→4)-β-D-Glc-(1→4)-β-D-Glc-(1→6)]-2(or3)-O-Ac-α-D-Glc-(1→6)]_n 4,6-O-(1-carboxyethan-1,1-diyl)transferase

Reaction

32087 [IUBMB]

[4)-β-D-GlcA-(1→4)-2-O-Ac-β-D-GlcA-(1→4)-β-D-Glc-(1→4)-[3-O-(CH₃CH(OH)CH₂C(O))-(R)-4,6-CH₃(COO⁻)C-β-D-Gal-(1→3)-β-D-Glc-(1→4)-β-D-Glc-(1→4)-β-D-Glc-(1→6)]-2-O-Ac-α-DGlc-(1→]_(n)

[4)-beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-(R)-4,6-CH3(COO(-))C-beta-D-Gal-(1->3)-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2-O-Ac-alpha-DGlc-(1->](n)

POLYMER:14004

Formula: H2O(C59H85O48)_n
Charge: (0)(-3)_n

ChEBI polymer: CHEBI:138369

+
n

phosphoenolpyruvate

phosphoenolpyruvate

Name origin: UniProt - CHECKED (C)

CHEBI:58702

Formula: C3H2O6P
Charge: -3

ChEBI compound status: CHECKED (C)

=

[4)-β-D-GlcA-(1→4)-2-O-Ac-β-D-GlcA-(1→4)-β-D-Glc-(1→4)-[3-O-(CH₃CH(OH)CH₂C(O))-(R)-4,6-CH₃(COO⁻)C-β-D-Gal-(1→3)-(S)-4,6-CH₃(COO⁻)C-β-D-Glc-(1→4)-β-D-Glc-(1→4)-β-D-Glc-(1→6)]-2-O-Ac-α-DGlc-(1→]_(n)

[4)-beta-D-GlcA-(1->4)-2-O-Ac-beta-D-GlcA-(1->4)-beta-D-Glc-(1->4)-[3-O-(CH3CH(OH)CH2C(O))-(R)-4,6-CH3(COO(-))C-beta-D-Gal-(1->3)-(S)-4,6-CH3(COO(-))C-beta-D-Glc-(1->4)-beta-D-Glc-(1->4)-beta-D-Glc-(1->6)]-2-O-Ac-alpha-DGlc-(1->](n)

POLYMER:13379

Formula: H2O(C62H86O50)_n
Charge: (0)(-4)_n

ChEBI polymer: CHEBI:63679

+
n

phosphate

phosphate

Name origin: UniProt - CHECKED (C)

CHEBI:43474

Formula: HO4P
Charge: -2

ChEBI compound status: CHECKED (C)

Comments:

The enzyme is responsible for pyruvylation of subterminal glucose in the acidic octasaccharide repeating unit of the exopolysaccharide of Rhizobium leguminosarum (bv. viciae strain VF39) which is necessary to establish nitrogen-fixing symbiosis with Pisum sativum, Vicia faba, and Vicia sativa.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway

Structural data: CSA , EC2PDB

UniProtKB/Swiss-Prot:

Q9FCP2

PSSM_RHILV

References

Ivashina, T. V., Fedorova, E. E., Ashina, N. P., Kalinchuk, N. A., Druzhinina, T. N., Shashkov, A. S., Shibaev, V. N., Ksenzenko, V. N.

Mutation in the pssM gene encoding ketal pyruvate transferase leads to disruption of Rhizobium leguminosarum bv. viciae-Pisum sativum symbiosis.

J. Appl. Microbiol. 109 : 731-742 (2010). [PMID: 20233262]

[EC 2.5.1.98 created 2012, modified 2018]

EC 2 - Transferases