EC - 6,7-dimethyl-8-ribityllumazine synthase

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IntEnz Enzyme Nomenclature


Accepted name:
6,7-dimethyl-8-ribityllumazine synthase
Other names:
lumazine synthase
6,7-dimethyl-8-ribityllumazine synthase 2
6,7-dimethyl-8-ribityllumazine synthase 1
lumazine synthase 2
lumazine synthase 1
type I lumazine synthase
type II lumazine synthase
RibH1 protein
RibH2 protein
Systematic name:
5-amino-6-(D-ribitylamino)uracil butanedionetransferase



Involved in riboflavin biosynthesis.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0000906
UniProtKB/Swiss-Prot: (616) [show] [UniProt]


  1. Kis, K., Volk, R., Bacher, A.
    Biosynthesis of riboflavin. Studies on the reaction mechanism of 6,7-dimethyl-8-ribityllumazine synthase.
    Biochemistry 34 : 2883-2892 (1995). [PMID: 7893702]
  2. Garcia-Ramirez, J. J., Santos, M. A., Revuelta, J. L.
    The Saccharomyces cerevisiae RIB4 gene codes for 6,7-dimethyl-8-ribityllumazine synthase involved in riboflavin biosynthesis. Molecular characterization of the gene and purification of the encoded protein.
    J. Biol. Chem. 270 : 23801-23807 (1995). [PMID: 7559556]
  3. Bacher, A., Fischer, M., Kis, K., Kugelbrey, K., Mortl, S., Scheuring, J., Weinkauf, S., Eberhardt, S., Schmidt-Base, K., Huber, R., Ritsert, K., Cushman, M., Ladenstein, R.
    Biosynthesis of riboflavin: structure and mechanism of lumazine synthase.
    Biochem. Soc. Trans. 24 : 89-94 (1996). [PMID: 8674771]
  4. Mortl, S., Fischer, M., Richter, G., Tack, J., Weinkauf, S., Bacher, A.
    Biosynthesis of riboflavin. Lumazine synthase of Escherichia coli.
    J. Biol. Chem. 271 : 33201-33207 (1996). [PMID: 8969176]
  5. Bacher, A., Eberhardt, S., Fischer, M., Mortl, S., Kis, K., Kugelbrey, K., Scheuring, J., Schott, K.
    Biosynthesis of riboflavin: lumazine synthase and riboflavin synthase.
    Meth. Enzymol. 280 : 389-399 (1997). [PMID: 9211334]
  6. Goldbaum, F. A., Velikovsky, C. A., Baldi, P. C., Mortl, S., Bacher, A., Fossati, C. A.
    The 18-kDa cytoplasmic protein of Brucella species --an antigen useful for diagnosis--is a lumazine synthase.
    J. Med. Microbiol. 48 : 833-839 (1999). [PMID: 10482294]
  7. Jordan, D. B., Bacot, K. O., Carlson, T. J., Kessel, M., Viitanen, P. V.
    Plant riboflavin biosynthesis. Cloning, chloroplast localization, expression, purification, and partial characterization of spinach lumazine synthase.
    J. Biol. Chem. 274 : 22114-22121 (1999). [PMID: 10419541]
  8. Zhang, X., Meining, W., Fischer, M., Bacher, A., Ladenstein, R.
    X-ray structure analysis and crystallographic refinement of lumazine synthase from the hyperthermophile Aquifex aeolicus at 1.6 Å resolution: determinants of thermostability revealed from structural comparisons.
    J. Mol. Biol. 306 : 1099-1114 (2001). [PMID: 11237620]
  9. Fischer, M., Haase, I., Feicht, R., Richter, G., Gerhardt, S., Changeux, J. P., Huber, R., Bacher, A.
    Biosynthesis of riboflavin: 6,7-dimethyl-8-ribityllumazine synthase of Schizosaccharomyces pombe.
    Eur. J. Biochem. 269 : 519-526 (2002). [PMID: 11856310]
  10. Cushman, M., Yang, D., Gerhardt, S., Huber, R., Fischer, M., Kis, K., Bacher, A.
    Design, synthesis, and evaluation of 6-carboxyalkyl and 6-phosphonoxyalkyl derivatives of 7-oxo-8-ribitylaminolumazines as inhibitors of riboflavin synthase and lumazine synthase.
    J. Org. Chem. 67 : 5807-5816 (2002). [PMID: 12153285]
  11. Haase, I., Mortl, S., Kohler, P., Bacher, A., Fischer, M.
    Biosynthesis of riboflavin in archaea. 6,7-dimethyl-8-ribityllumazine synthase of Methanococcus jannaschii.
    Eur. J. Biochem. 270 : 1025-1032 (2003). [PMID: 12603336]
  12. Morgunova, E., Meining, W., Illarionov, B., Haase, I., Jin, G., Bacher, A., Cushman, M., Fischer, M., Ladenstein, R.
    Crystal structure of lumazine synthase from Mycobacterium tuberculosis as a target for rational drug design: binding mode of a new class of purinetrione inhibitors.
    Biochemistry 44 : 2746-2758 (2005). [PMID: 15723519]

[EC created 2010]