EC 2.4.99.13 - (Kdo)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase

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IntEnz Enzyme Nomenclature
EC 2.4.99.13

Names

Accepted name:
(Kdo)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase
Other names:
waaA
kdtA
Kdo transferase
3-deoxy-D-manno-oct-2-ulosonic acid transferase
3-deoxy-manno-octulosonic acid transferase
Systematic name:
CMP-3-deoxy-β-D-manno-oct-2-ulosonate:α-Kdo-(2→6)-[lipid IVA] 3-deoxy-D-manno-oct-2-ulosonate transferase (configuration-inverting)

Reactions

Comments:

The enzyme from Escherichia coli is bifunctional and transfers two 3-deoxy-D-manno-oct-2-ulosonate residues to lipid IVA (cf. EC 2.4.99.12 [lipid IVA 3-deoxy-D-manno-octulosonic acid transferase]) [1]. The enzymes from Chlamydia transfer three or more 3-deoxy-D-manno-oct-2-ulosonate residues and generate genus-specific epitopes [2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0043842
UniProtKB/Swiss-Prot: (10) [show] [UniProt]

References

  1. Belunis, C. J., Raetz, C. R.
    Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli.
    J. Biol. Chem. 267 : 9988-9997 (1992). [PMID: 1577828]
  2. Lobau, S., Mamat, U., Brabetz, W., Brade, H.
    Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-α-D-manno-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183.
    Mol. Microbiol. 18 : 391-399 (1995). [PMID: 8748024]
  3. Schmidt, H., Hansen, G., Singh, S., Hanuszkiewicz, A., Lindner, B., Fukase, K., Woodard, R. W., Holst, O., Hilgenfeld, R., Mamat, U., Mesters, J. R.
    Structural and mechanistic analysis of the membrane-embedded glycosyltransferase WaaA required for lipopolysaccharide synthesis.
    Proc Natl Acad Sci U S A 109 : 6253-6258 (2012). [PMID: 22474366]

[EC 2.4.99.13 created 2010, modified 2011]