IntEnz

EC 2.4.99.13 - (Kdo)-lipid IV_A 3-deoxy-D-manno-octulosonic acid transferase

IntEnz Enzyme Nomenclature
EC 2.4.99.13

Names

Reaction

• 28062 [IUBMB]
  α-Kdo-(2→6)-lipid IV_A

  alpha-Kdo-(2->6)-lipid IVA

  Name origin: UniProt - CHECKED (C)

  CHEBI:60364

  Formula: C76H137N2O30P2
  Charge: -5

  ChEBI compound status: CHECKED (C)

  

  +

  CMP-3-deoxy-β-D-manno-octulosonate

  CMP-3-deoxy-beta-D-manno-octulosonate

  Name origin: UniProt - CHECKED (C)

  CHEBI:85987

  Formula: C17H24N3O15P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  =

  α-Kdo-(2→4)-α-Kdo-(2→6)-lipid IV_A

  alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA

  Name origin: UniProt - CHECKED (C)

  CHEBI:60365

  Formula: C84H148N2O37P2
  Charge: -6

  ChEBI compound status: CHECKED (C)

  

  +

  CMP

  CMP

  Name origin: UniProt - CHECKED (C)

  CHEBI:60377

  Formula: C9H12N3O8P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

Comments:

The bifunctional enzyme from Escherichia coli transfers two 3-deoxy-D-manno-octulosonate residues to lipid IV_A (cf. EC 2.4.99.12 [lipid IV_A 3-deoxy-D-manno-octulosonic acid transferase]) [1]. The enzymes from Chlamydia transfer three or more 3-deoxy-D-manno-octulosonate residues and generate genus-specific epitopes [4].

Links to other databases

References

1. Belunis, C. J., Raetz, C. R.
   Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli.
   J. Biol. Chem. 267: 9988-9997 (1992). [PMID: 1577828]
2. Lobau, S., Mamat, U., Brabetz, W., Brade, H.
   Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-α-D-manno-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183.
   Mol. Microbiol. 18: 391-399 (1995). [PMID: 8748024]

[EC 2.4.99.13 created 2010, modified 2011]