EC 2.4.99.12 - Lipid IVA 3-deoxy-D-manno-octulosonic acid transferase

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IntEnz Enzyme Nomenclature
EC 2.4.99.12

Names

Accepted name:
lipid IVA 3-deoxy-D-manno-octulosonic acid transferase
Other names:
KDO transferase
waaA
kdtA
3-deoxy-D-manno-oct-2-ulosonic acid transferase
3-deoxy-manno-octulosonic acid transferase
lipid IVA KDO transferase
Systematic name:
CMP-3-deoxy-β-D-manno-oct-2-ulosonate:lipid IVA 3-deoxy-β-D-manno-oct-2-ulosonate transferase (configuration-inverting)

Reactions

Comments:

The enzyme from Escherichia coli is bifunctional and transfers two 3-deoxy-D-manno-oct-2-ulosonate residues to lipid IVA (cf. EC 2.4.99.13 [(Kdo)-lipid IVA 3-deoxy-D-manno-octulosonic acid transferase]) [1]. The monofunctional enzymes from Bordetella pertusis, Aquifex aeolicus and Haemophilus influenzae catalyse the transfer of a single 3-D-manno-oct-2-ulosonate residue from CMP-3-D-manno-oct-2-ulosonate to lipid IVA [2-4]. The enzymes from Chlamydia transfer three or more 3-deoxy-D-manno-oct-2-ulosonate residues and generate genus-specific epitopes [5].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0043842
UniProtKB/Swiss-Prot: (18) [show] [UniProt]

References

  1. Belunis, C. J., Raetz, C. R.
    Biosynthesis of endotoxins. Purification and catalytic properties of 3-deoxy-D-manno-octulosonic acid transferase from Escherichia coli.
    J. Biol. Chem. 267 : 9988-9997 (1992). [PMID: 1577828]
  2. Mamat, U., Schmidt, H., Munoz, E., Lindner, B., Fukase, K., Hanuszkiewicz, A., Wu, J., Meredith, T. C., Woodard, R. W., Hilgenfeld, R., Mesters, J. R., Holst, O.
    WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis.
    J. Biol. Chem. 284 : 22248-22262 (2009). [PMID: 19546212]
  3. White, K. A., Kaltashov, I. A., Cotter, R. J., Raetz, C. R.
    A mono-functional 3-deoxy-D-manno-octulosonic acid (Kdo) transferase and a Kdo kinase in extracts of Haemophilus influenzae.
    J. Biol. Chem. 272 : 16555-16563 (1997). [PMID: 9195966]
  4. Lobau, S., Mamat, U., Brabetz, W., Brade, H.
    Molecular cloning, sequence analysis, and functional characterization of the lipopolysaccharide biosynthetic gene kdtA encoding 3-deoxy-alpha-D-manno-octulosonic acid transferase of Chlamydia pneumoniae strain TW-183.
    Mol. Microbiol. 18 : 391-399 (1995). [PMID: 8748024]
  5. Isobe, T., White, K. A., Allen, A. G., Peacock, M., Raetz, C. R., Maskell, D. J.
    Bordetella pertussis waaA encodes a monofunctional 2-keto-3-deoxy-D-manno-octulosonic acid transferase that can complement an Escherichia coli waaA mutation.
    J Bacteriol 181 : 2648-2651 (1999). [PMID: 10198035]

[EC 2.4.99.12 created 2010, updated 2011]