IntEnz

EC 2.4.2.52 - Triphosphoribosyl-dephospho-CoA synthase

IntEnz Enzyme Nomenclature
EC 2.4.2.52

Names

Reaction

• ATP + 3'-dephospho-CoA = 2'-(5-triphospho-alpha-D-ribosyl)-3'-dephospho-CoA + adenine

Comments:

ATP cannot be replaced by GTP, CTP, UTP, ADP or AMP. The reaction involves the formation of a new α (1''→2') glycosidic bond between the two ribosyl moieties, with concomitant displacement of the adenine moiety of ATP [1,4]. The 2'-(5-triphosphoribosyl)-3'-dephospho-CoA produced can be transferred by EC 2.7.7.61, citrate lyase holo-[acyl-carrier protein] synthase, to the apo-acyl-carrier protein subunit (γ-subunit) of EC 4.1.3.6, citrate (pro-3S) lyase, thus converting it from an apo-enzyme into a holo-enzyme [1,3]. Alternatively, it can be transferred to the apo-ACP subunit of malonate decarboxylase by the action of EC 2.7.7.66, malonate decarboxylase holo-[acyl-carrier protein] synthase [4].

Links to other databases

References

1. Schneider, K., Dimroth, P. and Bott, M.
   Biosynthesis of the prosthetic group of citrate lyase.
   Biochemistry 39 : 9438-9450 (2000). [PMID: 10924139]
2. Schneider, K., Dimroth, P. and Bott, M.
   Identification of triphosphoribosyl-dephospho-CoA as precursor of the citrate lyase prosthetic group.
   FEBS Lett. 483 : 165-168 (2000). [PMID: 11042274]
3. Schneider, K., Kastner, C.N., Meyer, M., Wessel, M., Dimroth, P. and Bott, M.
   Identification of a gene cluster in Klebsiella pneumoniae which includes citX, a gene required for biosynthesis of the citrate lyase prosthetic group.
   J. Bacteriol. 184 : 2439-2446 (2002). [PMID: 11948157]
4. Hoenke, S., Wild, M.R. and Dimroth, P.
   Biosynthesis of triphosphoribosyl-dephospho-coenzyme A, the precursor of the prosthetic group of malonate decarboxylase.
   Biochemistry 39 : 13223-13232 (2000). [PMID: 11052675]

[EC 2.4.2.52 created 2002 as EC 2.7.8.25, modified 2008, transferred 2013 to EC 2.4.2.52]