EC 2.4.2.2 - Pyrimidine-nucleoside phosphorylase
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IntEnz Enzyme Nomenclature
EC 2.4.2.2
Names
Accepted name:
pyrimidine-nucleoside phosphorylase
Other
names:
Py-NPase
pdp (gene name)
pdp (gene name)
Systematic name:
pyrimidine-nucleoside:phosphate (2'-deoxy)-α-D-ribosyltransferase
Reactions
- (1) uridine + phosphate = uracil + alpha-D-ribose 1-phosphate
- (2) cytidine + phosphate = cytosine + alpha-D-ribose 1-phosphate
- (3) 2'-deoxyuridine + phosphate = uracil + 2-deoxy-alpha-D-ribose 1-phosphate
- (4) thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate
Comments:
Unlike EC 2.4.2.3, uridine phosphorylase, EC 2.4.2.4, and thymidine phosphorylase, this enzyme can accept both the ribonucleosides uridine and cytidine and the 2'-deoxyribonucleosides 2'-deoxyuridine and thymidine [3,6]. The reaction is reversible, and the enzyme does not distinguish between α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate in the synthetic direction.
Links to other databases
Protein domains and families:
PROSITE:PDOC00557
Gene Ontology:
GO:0016154
CAS Registry Number:
9055-35-0
References
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Nucleoside phosphorylases.In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Eds.) The Enzymes , 2nd ed. vol. 5 , Academic Press , New York , 1961 , 237-255
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Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus.J. Biol. Chem. 244 : 3691-3697 (1969). [PMID: 4978445]
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Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2.Biosci. Biotechnol. Biochem. 60 : 1179-1180 (1996). [PMID: 8782414]
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Molecular cloning and expression of the pyrimidine nucleoside phosphorylase gene from Bacillus stearothermophilus TH 6-2.Biosci. Biotechnol. Biochem. 60 : 1655-1659 (1996). [PMID: 8987664]
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The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.Structure 6 : 1467-1479 (1998). [PMID: 9817849]
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Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside.J Zhejiang Univ Sci B 9 : 520-526 (2008). [PMID: 18600781]
[EC 2.4.2.2 created 1961, modified 2013]