IntEnz

EC 2.4.2.2 - Pyrimidine-nucleoside phosphorylase

IntEnz Enzyme Nomenclature
EC 2.4.2.2

Names

Reactions

• 16037 [IUBMB]
  phosphate

  phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:43474

  Formula: HO4P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  thymidine

  thymidine

  Name origin: UniProt - CHECKED (C)

  CHEBI:17748

  Formula: C10H14N2O5
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  =

  2-deoxy-α-D-ribose 1-phosphate

  2-deoxy-alpha-D-ribose 1-phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:57259

  Formula: C5H9O7P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  thymine

  thymine

  Name origin: UniProt - CHECKED (C)

  CHEBI:17821

  Formula: C5H6N2O2
  Charge: 0

  ChEBI compound status: CHECKED (C)

  
• 22824 [IUBMB]
  2'-deoxyuridine

  2'-deoxyuridine

  Name origin: UniProt - CHECKED (C)

  CHEBI:16450

  Formula: C9H12N2O5
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  +

  phosphate

  phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:43474

  Formula: HO4P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  =

  2-deoxy-α-D-ribose 1-phosphate

  2-deoxy-alpha-D-ribose 1-phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:57259

  Formula: C5H9O7P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  uracil

  uracil

  Name origin: UniProt - CHECKED (C)

  CHEBI:17568

  Formula: C4H4N2O2
  Charge: 0

  ChEBI compound status: CHECKED (C)

  
• 24388 [IUBMB]
  phosphate

  phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:43474

  Formula: HO4P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  uridine

  uridine

  Name origin: UniProt - CHECKED (C)

  CHEBI:16704

  Formula: C9H12N2O6
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

  =

  α-D-ribose 1-phosphate

  alpha-D-ribose 1-phosphate

  Name origin: UniProt - CHECKED (C)

  CHEBI:57720

  Formula: C5H9O8P
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  +

  uracil

  uracil

  Name origin: UniProt - CHECKED (C)

  CHEBI:17568

  Formula: C4H4N2O2
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

Comments:

Unlike EC 2.4.2.3, uridine phosphorylase, EC 2.4.2.4, and thymidine phosphorylase, this enzyme can accept both the ribonucleoside uridine and the 2'-deoxyribonucleosides 2'-deoxyuridine and thymidine [3]. The reaction is reversible, and the enzyme does not distinguish between α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate in the synthetic direction.

Links to other databases

References

1. Friedkin, M. and Kalckar, H.
   Nucleoside phosphorylases.
   In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Eds.) The Enzymes , 2nd ed. vol. 5 , Academic Press , New York , 1961 , 237-255
2. Saunders, P.P., Wilson, B.A. and Saunders, G.F.
   Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus.
   J. Biol. Chem. 244 : 3691-3697 (1969). [PMID: 4978445]
3. Hamamoto, T., Noguchi, T. and Midorikawa, Y.
   Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2.
   Biosci. Biotechnol. Biochem. 60 : 1179-1180 (1996). [PMID: 8782414]
4. Okuyama, K., Hamamoto, T., Noguchi, T., Midorikawa, Y.
   Molecular cloning and expression of the pyrimidine nucleoside phosphorylase gene from Bacillus stearothermophilus TH 6-2.
   Biosci. Biotechnol. Biochem. 60 : 1655-1659 (1996). [PMID: 8987664]
5. Pugmire, M. J., Ealick, S. E.
   The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.
   Structure 6 : 1467-1479 (1998). [PMID: 9817849]

[EC 2.4.2.2 created 1961, modified 2013]