EC 2.4.2.2 - Pyrimidine-nucleoside phosphorylase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 2.4.2.2
Names
Accepted name:
pyrimidine-nucleoside phosphorylase
Other
names:
Py-NPase
pdp (gene name)
pdp (gene name)
Systematic name:
pyrimidine-nucleoside:phosphate (2'-deoxy)-α-D-ribosyltransferase
Reactions
-
16037 [IUBMB]phosphateName origin: UniProt - CHECKED (C)Formula: HO4P
Charge: -2ChEBI compound status: CHECKED (C)thymidineName origin: UniProt - CHECKED (C)Formula: C10H14N2O5
Charge: 0ChEBI compound status: CHECKED (C)=2-deoxy-α-D-ribose 1-phosphateName origin: UniProt - CHECKED (C)Formula: C5H9O7P
Charge: -2ChEBI compound status: CHECKED (C) -
22824 [IUBMB]2'-deoxyuridineName origin: UniProt - CHECKED (C)Formula: C9H12N2O5
Charge: 0ChEBI compound status: CHECKED (C)phosphateName origin: UniProt - CHECKED (C)Formula: HO4P
Charge: -2ChEBI compound status: CHECKED (C)=2-deoxy-α-D-ribose 1-phosphateName origin: UniProt - CHECKED (C)Formula: C5H9O7P
Charge: -2ChEBI compound status: CHECKED (C) -
24388 [IUBMB]phosphateName origin: UniProt - CHECKED (C)Formula: HO4P
Charge: -2ChEBI compound status: CHECKED (C)uridineName origin: UniProt - CHECKED (C)Formula: C9H12N2O6
Charge: 0ChEBI compound status: CHECKED (C)=α-D-ribose 1-phosphateName origin: UniProt - CHECKED (C)Formula: C5H9O8P
Charge: -2ChEBI compound status: CHECKED (C)
Comments:
Unlike EC 2.4.2.3, uridine phosphorylase, EC 2.4.2.4, and thymidine phosphorylase, this enzyme can accept both the ribonucleoside uridine and the 2'-deoxyribonucleosides 2'-deoxyuridine and thymidine [3]. The reaction is reversible, and the enzyme does not distinguish between α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate in the synthetic direction.
Links to other databases
Enzymes and pathways:
NC-IUBMB
,
BRENDA
,
ExplorEnz
,
ENZYME@ExPASy
,
KEGG
,
MetaCyc
,
NIST 74
,
UniPathway
Protein domains and families:
PROSITE:PDOC00557
Gene Ontology:
GO:0016154
CAS Registry Number:
9055-35-0
References
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Nucleoside phosphorylases.In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Eds.) The Enzymes , 2nd ed. vol. 5 , Academic Press , New York , 1961 , 237-255
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Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus.J. Biol. Chem. 244 : 3691-3697 (1969). [PMID: 4978445]
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Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2.Biosci. Biotechnol. Biochem. 60 : 1179-1180 (1996). [PMID: 8782414]
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Molecular cloning and expression of the pyrimidine nucleoside phosphorylase gene from Bacillus stearothermophilus TH 6-2.Biosci. Biotechnol. Biochem. 60 : 1655-1659 (1996). [PMID: 8987664]
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The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.Structure 6 : 1467-1479 (1998). [PMID: 9817849]
[EC 2.4.2.2 created 1961, modified 2013]