EC 2.4.2.2 - Pyrimidine-nucleoside phosphorylase

  IntEnz view ENZYME view
XML

IntEnz Enzyme Nomenclature
EC 2.4.2.2

Names

Accepted name:
pyrimidine-nucleoside phosphorylase
Other names:
Py-NPase
pdp (gene name)
Systematic name:
pyrimidine-nucleoside:phosphate (2'-deoxy)-α-D-ribosyltransferase

Reactions

Comments:

Unlike EC 2.4.2.3, uridine phosphorylase, EC 2.4.2.4, and thymidine phosphorylase, this enzyme can accept both the ribonucleosides uridine and cytidine and the 2'-deoxyribonucleosides 2'-deoxyuridine and thymidine [3,6]. The reaction is reversible, and the enzyme does not distinguish between α-D-ribose 1-phosphate and 2-deoxy-α-D-ribose 1-phosphate in the synthetic direction.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00557
Structural data: CSA , EC2PDB
Gene Ontology: GO:0016154
CAS Registry Number: 9055-35-0
UniProtKB/Swiss-Prot: (201) [show] [UniProt]

References

  1. Friedkin, M. and Kalckar, H.
    Nucleoside phosphorylases.
    In: Boyer, P.D., Lardy, H. and Myrbäck, K. (Eds.) The Enzymes , 2nd ed. vol. 5 , Academic Press , New York , 1961 , 237-255
  2. Saunders, P.P., Wilson, B.A. and Saunders, G.F.
    Purification and comparative properties of a pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus.
    J. Biol. Chem. 244 : 3691-3697 (1969). [PMID: 4978445]
  3. Hamamoto, T., Noguchi, T. and Midorikawa, Y.
    Purification and characterization of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase from Bacillus stearothermophilus TH 6-2.
    Biosci. Biotechnol. Biochem. 60 : 1179-1180 (1996). [PMID: 8782414]
  4. Okuyama, K., Hamamoto, T., Noguchi, T., Midorikawa, Y.
    Molecular cloning and expression of the pyrimidine nucleoside phosphorylase gene from Bacillus stearothermophilus TH 6-2.
    Biosci. Biotechnol. Biochem. 60 : 1655-1659 (1996). [PMID: 8987664]
  5. Pugmire, M. J., Ealick, S. E.
    The crystal structure of pyrimidine nucleoside phosphorylase in a closed conformation.
    Structure 6 : 1467-1479 (1998). [PMID: 9817849]
  6. Wei, X. K., Ding, Q. B., Zhang, L., Guo, Y. L., Ou, L., Wang, C. L.
    Induction of nucleoside phosphorylase in Enterobacter aerogenes and enzymatic synthesis of adenine arabinoside.
    J Zhejiang Univ Sci B 9 : 520-526 (2008). [PMID: 18600781]

[EC 2.4.2.2 created 1961, modified 2013]