EC 2.4.1.289 - N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase

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IntEnz Enzyme Nomenclature
EC 2.4.1.289

Names

Accepted name:
N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase
Other name:
WbbL
Systematic name:
dTDP-6-deoxy-β-L-mannose:N-acetyl-α-D-glucosaminyl-diphospho-trans,octacis-decaprenol 3-α-L-rhamnosyltransferase

Reaction

Cofactor

Comments:

Requires Mn2+ or Mg2+. Isolated from Mycobacterium smegmatis [1] and Mycobacterium tuberculosis [2]. The enzyme catalyses the addition of a rhamnosyl unit to N-acetyl-α-D-glucosaminyl-diphospho-trans,octacis-decaprenol, completing the synthesis of the linkage unit that attaches the arabinogalactan moiety to the peptidoglycan moiety in Mycobacterial cell wall.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102096
UniProtKB/Swiss-Prot:

References

  1. Mills, J. A., Motichka, K., Jucker, M., Wu, H. P., Uhlik, B. C., Stern, R. J., Scherman, M. S., Vissa, V. D., Pan, F., Kundu, M., Ma, Y. F., McNeil, M.
    Inactivation of the mycobacterial rhamnosyltransferase, which is needed for the formation of the arabinogalactan-peptidoglycan linker, leads to irreversible loss of viability.
    J. Biol. Chem. 279: 43540-43546 (2004). [PMID: 15294902]
  2. Grzegorzewicz, A. E., Ma, Y., Jones, V., Crick, D., Liav, A., McNeil, M. R.
    Development of a microtitre plate-based assay for lipid-linked glycosyltransferase products using the mycobacterial cell wall rhamnosyltransferase WbbL.
    Microbiology (Reading, Engl.) 154: 3724-3730 (2008). [PMID: 19047740]

[EC 2.4.1.289 created 2012]