EC - Glucosyl-3-phosphoglycerate synthase

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IntEnz Enzyme Nomenclature


Accepted name:
glucosyl-3-phosphoglycerate synthase
Other names:
GpgS protein
GPG synthase
glucosylphosphoglycerate synthase
Systematic name:
NDP-glucose:3-phospho-D-glycerate 2-α-D-glucosyltransferase



The enzyme is involved in biosynthesis of 2-O-(α-D-glucopyranosyl)-D-glycerate via the two-step pathway in which glucosyl-3-phosphoglycerate synthase catalyses the conversion of GDP-glucose and 3-phospho-D-glycerate into 2-O-(α-D-glucopyranosyl)-3-phospho-D-glycerate, which is then converted to 2-O-(α-D-glucopyranosyl)-D-glycerate by EC glucosyl-3-phosphoglycerate phosphatase. The activity is dependent on divalent cations (Mn2+, Co2+, or Mg2+). The enzyme from Persephonella marina shows moderate flexibility on the sugar donor concerning the nucleotide moiety (UDP-glucose, ADP-glucose, GDP-glucose) but is strictly specific for glucose. The enzyme is also strictly specific for 3-phospho-D-glycerate as acceptor [1]. The enzyme from Methanococcoides burtonii is strictly specific for GDP-glucose and 3-phospho-D-glycerate [2]. This enzyme catalyses the first glucosylation step in methylglucose lipopolysaccharide biosynthesis in mycobacteria [4,5].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Costa, J., Empadinhas, N., da Costa, M. S.
    Glucosylglycerate biosynthesis in the deepest lineage of the Bacteria: characterization of the thermophilic proteins GpgS and GpgP from Persephonella marina.
    J. Bacteriol. 189: 1648-1654 (2007). [PMID: 17189358]
  2. Costa, J., Empadinhas, N., Goncalves, L., Lamosa, P., Santos, H., da Costa, M. S.
    Characterization of the biosynthetic pathway of glucosylglycerate in the archaeon Methanococcoides burtonii.
    J. Bacteriol. 188: 1022-1030 (2006). [PMID: 16428406]
  3. Empadinhas, N., Albuquerque, L., Mendes, V., Macedo-Ribeiro, S., da Costa, M. S.
    Identification of the mycobacterial glucosyl-3-phosphoglycerate synthase.
    FEMS Microbiol. Lett. 280: 195-202 (2008). [PMID: 18221489]
  4. Pereira, P. J., Empadinhas, N., Albuquerque, L., Sa-Moura, B., da Costa, M. S., Macedo-Ribeiro, S.
    Mycobacterium tuberculosis glucosyl-3-phosphoglycerate synthase: structure of a key enzyme in methylglucose lipopolysaccharide biosynthesis.
    PLoS ONE 3: e3748 (2008). [PMID: 19015727]
  5. Gest, P., Kaur, D., Pham, H. T., van der Woerd, M., Hansen, E., Brennan, P. J., Jackson, M., Guerin, M. E.
    Preliminary crystallographic analysis of GpgS, a key glucosyltransferase involved in methylglucose lipopolysaccharide biosynthesis in Mycobacterium tuberculosis.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64: 1121-1124 (2008). [PMID: 19052364]
  6. Kaur, D., Pham, H., Larrouy-Maumus, G., Riviere, M., Vissa, V., Guerin, M. E., Puzo, G., Brennan, P. J., Jackson, M.
    Initiation of methylglucose lipopolysaccharide biosynthesis in mycobacteria.
    PLoS ONE 4: e5447 (2009). [PMID: 19421329]

[EC created 2011]