EC 2.4.1.264 - D-Man-α-(1→3)-D-Glc-β-(1→4)-D-Glc-α-1-diphosphoundecaprenol 2-β-glucuronosyltransferase

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IntEnz Enzyme Nomenclature
EC 2.4.1.264

Names

Accepted name:
D-Man-α-(1→3)-D-Glc-β-(1→4)-D-Glc-α-1-diphosphoundecaprenol 2-β-glucuronosyltransferase
Other names:
GumK
UDP-glucuronate:D-Man-α-(1→3)-D-Glc-β-(1→4)-D-Glc-α-1-diphospho-ditrans,octacis-undecaprenol β-1,2-glucuronyltransferase
D-Man-α-(1→3)-D-Glc-β-(1→4)-D-Glc-α-1-diphosphoundecaprenol 2-β-glucuronyltransferase
Systematic name:
UDP-α-D-glucuronate:α-D-Man-(1→3)-β-D-Glc-(1→4)-α-D-Glc-1-diphospho-ditrans,octacis-undecaprenol β-1,2-glucuronosyltransferase (configuration-inverting)

Reaction

Comments:

The enzyme is involved in the biosynthesis of the exopolysaccharides xanthan (in the bacterium Xanthomonas campestris) and acetan (in the bacterium Gluconacetobacter xylinus).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot:

References

  1. Katzen, F., Ferreiro, D. U., Oddo, C. G., Ielmini, M. V., Becker, A., Puhler, A., Ielpi, L.
    Xanthomonas campestris pv. campestris gum mutants: effects on xanthan biosynthesis and plant virulence.
    J. Bacteriol. 180: 1607-1617 (1998). [PMID: 9537354]
  2. Ielpi, L., Couso, R. O., Dankert, M. A.
    Sequential assembly and polymerization of the polyprenol-linked pentasaccharide repeating unit of the xanthan polysaccharide in Xanthomonas campestris.
    J. Bacteriol. 175: 2490-2500 (1993). [PMID: 7683019]
  3. Kim, S. Y., Kim, J. G., Lee, B. M., Cho, J. Y.
    Mutational analysis of the gum gene cluster required for xanthan biosynthesis in Xanthomonas oryzae pv oryzae.
    Biotechnol. Lett. 31: 265-270 (2009). [PMID: 18854951]
  4. Barreras, M., Bianchet, M. A., Ielpi, L.
    Crystallization and preliminary crystallographic characterization of GumK, a membrane-associated glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 62: 880-883 (2006). [PMID: 16946469]
  5. Barreras, M., Salinas, S. R., Abdian, P. L., Kampel, M. A., Ielpi, L.
    Structure and mechanism of GumK, a membrane-associated glucuronosyltransferase.
    J. Biol. Chem. 283: 25027-25035 (2008). [PMID: 18596046]
  6. Vojnov, A. A., Bassi, D. E., Daniels, M. J., Dankert, M. A.
    Biosynthesis of a substituted cellulose from a mutant strain of Xanthomonas campestris.
    Carbohydr. Res. 337: 315-326 (2002). [PMID: 11841812]
  7. Barreras, M., Abdian, P. L., Ielpi, L.
    Functional characterization of GumK, a membrane-associated β-glucuronosyltransferase from Xanthomonas campestris required for xanthan polysaccharide synthesis.
    Glycobiology 14: 233-241 (2004). [PMID: 14736729]

[EC 2.4.1.264 created 2011, modified 2016]