EC 2.4.1.229 - [Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase

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IntEnz Enzyme Nomenclature
EC 2.4.1.229

Names

Accepted name:
[Skp1-protein]-hydroxyproline N-acetylglucosaminyltransferase
Other names:
Skp1-HyPro GlcNAc-transferase
UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase
UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase
UDP-GlcNAc:hydroxyproline polypeptide GlcNAc-transferase
UDP-N-acetyl-D-glucosamine:[Skp1-protein]-hydroxyproline N-acetyl-D-glucosaminyl-transferase
Systematic name:
UDP-N-acetyl-α-D-glucosamine:[Skp1-protein]-trans-4-hydroxy-L-proline N-acetyl-α-D-glucosaminyl-transferase

Reaction

Cofactor

Comments:

Skp1 is a cytoplasmic and nuclear protein required for the ubiquitination of cell cycle regulatory proteins and transcriptional factors. In Dictyostelium Skp1 is modified by the linear pentasaccharide Galα1-6Galα1-L-Fucα1-2Galβ1-3GlcNAc, which is attached to a hydroxyproline residue at position 143. This enzyme catalyses the first step in the building up of the pentasaccharide by attaching an N-acetylglucosaminyl group to the hydroxyproline residue. It requires dithiothreitol and a divalent cation for activity.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0033830
UniProtKB/Swiss-Prot:

References

  1. van der Wel, H.,Morris, H.R., Panico, M., Paxton, T., Dell, A., Kaplan, L. and West, C.M.
    Molecular cloning and expression of a UDP-N-acetylglucosamine (GlcNAc):hydroxyproline polypeptide GlcNAc-transferase that modifies Skp1 in the cytoplasm of Dictyostelium.
    J. Biol. Chem. 277 : 46328-46337 (2002). [PMID: 12244115]
  2. Teng-umnuay, P., van der Wel, H. and West, C.M.
    Identification of a UDP-GlcNAc:Skp1-hydroxyproline GlcNAc-transferase in the cytoplasm of Dictyostelium.
    J. Biol. Chem. 274 : 36392-36402 (1999). [PMID: 10593934]
  3. West, C.M., van der Wel, H. and Gaucher, E.A.
    Complex glycosylation of Skp1 in Dictyostelium: implications for the modification of other eukaryotic cytoplasmic and nuclear proteins.
    Glycobiology 12 : 17R-27R (2002). [PMID: 11886837]

[EC 2.4.1.229 created 2003, modified 2013]