EC 2.4.1.214 - Glycoprotein 3-α-L-fucosyltransferase

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IntEnz Enzyme Nomenclature
EC 2.4.1.214

Names

Accepted name:
glycoprotein 3-α-L-fucosyltransferase
Other names:
GDP-L-Fuc:N-acetyl-β-D-glucosaminide α1,3-fucosyltransferase
GDP-L-Fuc:Asn-linked GlcNAc α1,3-fucosyltransferase
GDP-fucose:β-N-acetylglucosamine (Fuc to (Fucα1→6GlcNAc)-Asn-peptide) α1→3-fucosyltransferase
GDP-fucose:β-N-acetylglucosamine (Fuc to (Fuc-α1→6-GlcNAc)-Asn-peptide) α1→3-fucosyltransferase
GDP-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of 4-N-{N-acetyl-β-D-glucosaminyl-(1→2)-α-D-mannosyl-(1→3)-[N-acetyl-β-D-glucosaminyl-(1→2)-α-D-mannosyl-(1→6)]-β-D-mannosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→4)-N-acetyl-β-D-glucosaminyl}asparagine) 3-α-L-fucosyl-transferase
GDP-β-L-fucose:glycoprotein (L-fucose to asparagine-linked N-acetylglucosamine of N4-{N-acetyl-β-D-glucosaminyl-(1→2)-α-D-mannosyl-(1→3)-[N-acetyl-β-D-glucosaminyl-(1→2)-α-D-mannosyl-(1→6)]-β-D-mannosyl-(1→4)-N-acetyl-β-D-glucosaminyl-(1→4)-N-acetyl-β-D-glucosaminyl}asparagine) 3-α-L-fucosyl-transferase
Systematic name:
GDP-β-L-fucose:N4-{β-D-GlcNAc-(1→2)-α-D-Man-(1→3)-[β-D-GlcNAc-(1→2)-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc}-L-asparaginyl-[protein] 3-α-L-fucosyl-transferase (configuration-retaining)

Reaction

Cofactor

Comments:

Requires Mn2+. The enzyme transfers to N-linked oligosaccharide structures (N-glycans), generally with a specificity for N-glycans with one unsubstituted non-reducing terminal GlcNAc residue. This enzyme catalyses a reaction similar to that of EC 2.4.1.68, glycoprotein 6-α-L-fucosyltransferase, but transferring the L-fucosyl group from GDP-β-L-fucose to form an α1,3-linkage rather than an α1,6-linkage. The N-glycan products of this enzyme are present in plants, insects and some other invertebrates (e.g., Schistosoma, Haemonchus, Lymnaea).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0018392
CAS Registry Number: 68247-53-0
UniProtKB/Swiss-Prot:

References

  1. Wilson, I.B.H., Rendic, D., Freilinger, A., Dumic, J., Altmann, F., Mucha, J., Müller, S. and Hauser, M.-T.
    Cloning and expression of α1,3-fucosyltransferase homologues from Arabidopsis thaliana.
    Biochim. Biophys. Acta 1527 : 88-96 (2001). [PMID: 11420147]
  2. Fabini, G., Freilinger, A., Altmann, F. and Wilson, I.B.H.
    Identification of core α1,3-fucosylated glycans and cloning of the requisite fucosyltransferase cDNA from Drosophila melanogaster. Potential basis of the neural anti-horseradish peroxidase epitope.
    J. Biol. Chem. 276 : 28058-28067 (2001). [PMID: 11382750]
  3. Leiter, H., Mucha, J., Staudacher, E., Grimm, R., Glössl, J. and Altmann, F.
    Purification, cDNA cloning, and expression of GDP-L-Fuc:Asn-linked GlcNAc α1,3-fucosyltransferase from mung beans.
    J. Biol. Chem. 274 : 21830-21839 (1999). [PMID: 10419500]
  4. van Tetering, A., Schiphorst, W.E.C.M., van den Eijnden, D.H. and van Die, I.
    Characterization of core α1→3-fucosyltransferase from the snail Lymnaea stagnalis that is involved in the synthesis of complex type N-glycans.
    FEBS Lett. 461 : 311-314 (1999). [PMID: 10567717]
  5. Staudacher, E., Altmann, F., Glössl, J., März, L., Schachter, H., Kamerling, J.P., Hård, K. and Vliegenthart, J.F.G.
    GDP-fucose:β-N-acetylglucosamine (Fuc to (Fucα1→6GlcNAc)-Asn-peptide) α1→3-fucosyltransferase activity in honeybee (Apis mellifica) venom glands. The difucosylation of asparagine-bound N-acetylglucosamine.
    Eur. J. Biochem. 199 : 745-751 (1991). [PMID: 1868856]

[EC 2.4.1.214 created 2001]