IntEnz

EC 2.4.1.175 - Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-β-N-acetylgalactosaminyltransferase

IntEnz Enzyme Nomenclature
EC 2.4.1.175

Names

Reactions

• 20800 [IUBMB]
  3-O-(β-D-GlcA-(1→3)-β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein]

  3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein]

  GENERIC:14058

  Is ROOT: no
  ROOT compound: GENERIC:9624

  Number of residues: 1

  O3-(β-D-GlcA-(1→3)-β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-serine residue O(3)-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine residue Name origin: UniProt - CHECKED (C) CHEBI:138442 Formula: C40H60N2O33 Charge: -2 Position: undefined ChEBI compound status: CHECKED (C)

  +

  UDP-N-acetyl-α-D-galactosamine

  UDP-N-acetyl-alpha-D-galactosamine

  Name origin: UniProt - CHECKED (C)

  CHEBI:67138

  Formula: C17H25N3O17P2
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  =

  3-O-(β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein]

  3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein]

  GENERIC:14059

  Is ROOT: no
  ROOT compound: GENERIC:9624

  Number of residues: 1

  O3-(β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-serine residue O(3)-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine residue Name origin: UniProt - CHECKED (C) CHEBI:138443 Formula: C48H73N3O38 Charge: -2 Position: undefined ChEBI compound status: CHECKED (C)

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  UDP

  UDP

  Name origin: UniProt - CHECKED (C)

  CHEBI:58223

  Formula: C9H11N2O12P2
  Charge: -3

  ChEBI compound status: CHECKED (C)

  
• 55000 [IUBMB]
  3-O-(β-D-GlcA-(1→3)-[β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)]_(n)-β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein]

  3-O-(beta-D-GlcA-(1->3)-[beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein]

  POLYMER:14060

  Formula: C40H60N2O33(C14H20NO11)_n
  Charge: (-2)(-1)_n

  ChEBI polymer: CHEBI:138444

  

  +

  UDP-N-acetyl-α-D-galactosamine

  UDP-N-acetyl-alpha-D-galactosamine

  Name origin: UniProt - CHECKED (C)

  CHEBI:67138

  Formula: C17H25N3O17P2
  Charge: -2

  ChEBI compound status: CHECKED (C)

  

  =

  3-O-([β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)]_(n+1)-β-D-GalNAc-(1→4)-β-D-GlcA-(1→3)-β-D-Gal-(1→3)-β-D-Gal-(1→4)-β-D-Xyl)-L-seryl-[protein]

  3-O-([beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)](n+1)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein]

  POLYMER:14301

  Formula: C34H53N2O27(C14H20NO11)_n+1
  Charge: (-1)(-1)_n+1

  ChEBI polymer: CHEBI:138445

  

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  UDP

  UDP

  Name origin: UniProt - CHECKED (C)

  CHEBI:58223

  Formula: C9H11N2O12P2
  Charge: -3

  ChEBI compound status: CHECKED (C)

  

Comments:

Involved in the biosynthesis of chondroitin sulfate. The human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-β-glucuronosyltransferase (EC 2.4.1.226) activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase 'co-polymerases' can be found in Pasteurella multocida and Escherichia coli.

Links to other databases

References

1. Rohrmann, K., Niemann, R. and Buddecke, E.
   Two N-acetylgalactosaminyltransferases are involved in the biosynthesis of chondroitin sulfate.
   Eur. J. Biochem. 148 : 463-469 (1985). [PMID: 3922754]
2. Kitagawa, H., Uyama, T. and Sugahara, K.
   Molecular cloning and expression of a human chondroitin synthase.
   J. Biol. Chem. 276 : 38721-38726 (2001). [PMID: 11514575]
3. DeAngelis, P.L. and Padgett-McCue, A.J.
   Identification and molecular cloning of a chondroitin synthase from Pasteurella multocida type F.
   J. Biol. Chem. 275 : 24124-24129 (2000). [PMID: 10818104]
4. Ninomiya, T., Sugiura, N., Tawada, A., Sugimoto, K., Watanabe, H. and Kimata, K.
   Molecular cloning and characterization of chondroitin polymerase from Escherichia coli strain K4.
   J. Biol. Chem. 277 : 21567-21575 (2002). [PMID: 11943778]

[EC 2.4.1.175 created 1989, modified 2002]