EC 2.4.1.161 - Oligosaccharide 4-α-D-glucosyltransferase
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IntEnz Enzyme Nomenclature
EC 2.4.1.161
Names
Accepted name:
oligosaccharide 4-α-D-glucosyltransferase
Other
names:
1,4-α-glucan:1,4-α-glucan 4-α-glucosyltransferase
amylase III
1,4-α-D-glucan:1,4-α-D-glucan 4-α-D-glucosyltransferase
α-1,4-transglucosylase
amylase III
1,4-α-D-glucan:1,4-α-D-glucan 4-α-D-glucosyltransferase
α-1,4-transglucosylase
Systematic name:
(1→4)-α-D-glucan:(1→4)-α-D-glucan 4-α-D-glucosyltransferase
Reaction
- Transfers the non-reducing terminal alpha-D-glucose residue from a (1right4)-alpha-D-glucan to the 4-position of a free glucose or of a glucosyl residue at the non-reducing terminus of a (1right4)-alpha-D-glucan, thus bringing about the rearrangement of oligosaccharides
Comments:
The enzyme acts on amylose, amylopectin, glycogen and maltooligosaccharides. No detectable free glucose is formed, indicating the enzyme does not act as a hydrolase. The enzyme from the bacterium Cellvibrio japonicus has the highest activity with maltotriose as a donor, and also accepts maltose [3], while the enzyme from amoeba does not accept maltose [1,2]. Oligosaccharides with 1→6 linkages cannot function as donors, but can act as acceptors [3]. Unlike EC 2.4.1.25, 4-α-glucanotransferase, this enzyme can transfer only a single glucosyl residue.
Links to other databases
Gene Ontology:
GO:0033825
CAS Registry Number:
9000-92-4
UniProtKB/Swiss-Prot:
OL4AG_CELJU
References
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Separation and characterization of four different amylases of Entamoeba histolytica. I. Purification and properties.Biol. Chem. Hoppe-Seyler 367 : 161-167 (1986). [PMID: 2423097]
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Separation and characterization of four different amylases of Entamoeba histolytica. II. Characterization of amylases.Biol. Chem. Hoppe-Seyler 367 : 169-176 (1986). [PMID: 2423098]
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Structural enzymology of Cellvibrio japonicus Agd31B protein reveals alpha-transglucosylase activity in glycoside hydrolase family 31.J. Biol. Chem. 287 : 43288-43299 (2012). [PMID: 23132856]
[EC 2.4.1.161 created 1989, modified 2013]