EC - 2-methylcitrate synthase

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IntEnz Enzyme Nomenclature


Accepted name:
2-methylcitrate synthase
Other names:
2-methylcitrate oxaloacetate-lyase
methylcitrate synthase
methylcitrate synthetase
Systematic name:
propanoyl-CoA:oxaloacetate C-propanoyltransferase (thioester-hydrolysing, 1-carboxyethyl-forming)



The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme is distinct from EC, citrate (Si)-synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00422
Structural data: CSA , EC2PDB
Gene Ontology: GO:0050440
CAS Registry Number: 57827-78-8
UniProtKB/Swiss-Prot: (15) [show] [UniProt]


  1. Uchiyama, H. and Tabuchi, T.
    Properties of methylcitrate synthase from Candida lipolytica.
    Agric. Biol. Chem. 40 : 1411-1418 (1976).
  2. Textor, S., Wendisch, V.F., De Graaf, A.A., Muller, U., Linder, M.I., Linder, D. and Buckel, W.
    Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria.
    Arch. Microbiol. 168 : 428-436 (1997). [PMID: 9325432]
  3. Horswill, A.R. and Escalante-Semerena, J.C.
    Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle.
    J. Bacteriol. 181 : 5615-5623 (1999). [PMID: 10482501]
  4. Brock, M., Maerker, C., Schutz, A., Volker, U., Buckel, W.
    Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase.
    Eur. J. Biochem. 269 : 6184-6194 (2002). [PMID: 12473114]
  5. Domin, N., Wilson, D., Brock, M.
    Methylcitrate cycle activation during adaptation of Fusarium solani and Fusarium verticillioides to propionyl-CoA-generating carbon sources.
    Microbiology (Reading, Engl.) 155 : 3903-3912 (2009). [PMID: 19661181]

[EC created 1978 as EC, transferred 2002 to EC, modified 2015]