EC 22.214.171.124 - 2-methylcitrate synthase
IntEnz Enzyme Nomenclature
- propanoyl-CoA + H2O + oxaloacetate = (2S,3S)-2-hydroxybutane-1,2,3-tricarboxylate + CoA
The enzyme acts on acetyl-CoA, propanoyl-CoA, butanoyl-CoA and pentanoyl-CoA. The relative rate of condensation of acetyl-CoA and oxaloacetate is 140% of that of propanoyl-CoA and oxaloacetate, but the enzyme is distinct from EC 126.96.36.199, citrate (Si)-synthase. Oxaloacetate cannot be replaced by glyoxylate, pyruvate or 2-oxoglutarate.
Links to other databases
Properties of methylcitrate synthase from Candida lipolytica.Agric. Biol. Chem. 40 : 1411-1418 (1976).
Propionate oxidation in Escherichia coli: evidence for operation of a methylcitrate cycle in bacteria.Arch. Microbiol. 168 : 428-436 (1997). [PMID: 9325432]
Salmonella typhimurium LT2 catabolizes propionate via the 2-methylcitric acid cycle.J. Bacteriol. 181 : 5615-5623 (1999). [PMID: 10482501]
Oxidation of propionate to pyruvate in Escherichia coli. Involvement of methylcitrate dehydratase and aconitase.Eur. J. Biochem. 269 : 6184-6194 (2002). [PMID: 12473114]
Methylcitrate cycle activation during adaptation of Fusarium solani and Fusarium verticillioides to propionyl-CoA-generating carbon sources.Microbiology (Reading, Engl.) 155 : 3903-3912 (2009). [PMID: 19661181]
[EC 188.8.131.52 created 1978 as EC 184.108.40.206, transferred 2002 to EC 220.127.116.11, modified 2015]