EC - Acyl-CoA:acyl-CoA alkyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
acyl-CoA:acyl-CoA alkyltransferase
Other name:
oleA (gene name)
Systematic name:
acyl-CoA:acyl-CoA alkyltransferase [(2R)-2-alkyl-3-oxoalkanoate-forming]



The enzyme, found in certain bacterial species, catalyses a head-to-head non-decarboxylative Claisen condensation of two acyl-CoA molecules, resulting in formation of a 2-alkyl-3-oxoalkanoic acid. It is part of a pathway for the production of olefins.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Sukovich, D. J., Seffernick, J. L., Richman, J. E., Hunt, K. A., Gralnick, J. A., Wackett, L. P.
    Structure, function, and insights into the biosynthesis of a head-to-head hydrocarbon in Shewanella oneidensis strain MR-1.
    Appl. Environ. Microbiol. 76 : 3842-3849 (2010). [PMID: 20418444]
  2. Frias, J. A., Richman, J. E., Erickson, J. S., Wackett, L. P.
    Purification and characterization of OleA from Xanthomonas campestris and demonstration of a non-decarboxylative Claisen condensation reaction.
    J. Biol. Chem. 286 : 10930-10938 (2011). [PMID: 21266575]
  3. Goblirsch, B. R., Frias, J. A., Wackett, L. P., Wilmot, C. M.
    Crystal structures of Xanthomonas campestris OleA reveal features that promote head-to-head condensation of two long-chain fatty acids.
    Biochemistry 51 : 4138-4146 (2012). [PMID: 22524624]
  4. Goblirsch, B. R., Jensen, M. R., Mohamed, F. A., Wackett, L. P., Wilmot, C. M.
    Substrate Trapping in Crystals of the Thiolase OleA Identifies Three Channels That Enable Long Chain Olefin Biosynthesis.
    J. Biol. Chem. 291 : 26698-26706 (2016). [PMID: 27815501]

[EC created 2018]