EC - Cullin-RING-type E3 NEDD8 transferase

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IntEnz Enzyme Nomenclature


Accepted name:
cullin-RING-type E3 NEDD8 transferase
Other name:
RBX1 (gene name)
Systematic name:
S-[NEDD8-protein]-yl-[E2 NEDD8-conjugating enzyme]-L-cysteine:[cullin] [NEDD8-protein] transferase (isopeptide bond-forming; RING-type)



Some RING-type E3 ubiquitin transferase (EC are not able to bind a substrate protein directly. Instead, they form a complex with a cullin scaffold protein and a substrate recognition module, which is named CRL for Cullin-RING-Ligase. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. The transfer of NEDD8 from a NEDD8-specific E2 enzyme onto the cullin protein is a secondary function of the RING-type E3 ubiquitin transferase in the CRL complex. The process requires auxiliary factors that belong to the DCN1 (defective in cullin neddylation 1) family.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (16) [show] [UniProt]


  1. Kim, A. Y., Bommelje, C. C., Lee, B. E., Yonekawa, Y., Choi, L., Morris, L. G., Huang, G., Kaufman, A., Ryan, R. J., Hao, B., Ramanathan, Y., Singh, B.
    SCCRO (DCUN1D1) is an essential component of the E3 complex for neddylation.
    J. Biol. Chem. 283 : 33211-33220 (2008). [PMID: 18826954]
  2. Kurz, T., Chou, Y. C., Willems, A. R., Meyer-Schaller, N., Hecht, M. L., Tyers, M., Peter, M., Sicheri, F.
    Dcn1 functions as a scaffold-type E3 ligase for cullin neddylation.
    Mol. Cell 29 : 23-35 (2008). [PMID: 18206966]
  3. Scott, D. C., Monda, J. K., Grace, C. R., Duda, D. M., Kriwacki, R. W., Kurz, T., Schulman, B. A.
    A dual E3 mechanism for Rub1 ligation to Cdc53.
    Mol. Cell 39 : 784-796 (2010). [PMID: 20832729]
  4. Scott, D. C., Sviderskiy, V. O., Monda, J. K., Lydeard, J. R., Cho, S. E., Harper, J. W., Schulman, B. A.
    Structure of a RING E3 trapped in action reveals ligation mechanism for the ubiquitin-like protein NEDD8.
    Cell 157 : 1671-1684 (2014). [PMID: 24949976]
  5. Monda, J. K., Scott, D. C., Miller, D. J., Lydeard, J., King, D., Harper, J. W., Bennett, E. J., Schulman, B. A.
    Structural conservation of distinctive N-terminal acetylation-dependent interactions across a family of mammalian NEDD8 ligation enzymes.
    Structure 21 : 42-53 (2013). [PMID: 23201271]

[EC created 2017]