EC 2.3.2.16 - Lipid II:glycine glycyltransferase

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IntEnz Enzyme Nomenclature
EC 2.3.2.16

Names

Accepted name:
lipid II:glycine glycyltransferase
Other names:
N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:N6-glycine transferase
femX
alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine:glycine N6-glycyltransferase
Systematic name:
MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphospho-ditrans,octacis-undecaprenyl-GlcNAc:glycine N6-glycyltransferase

Reaction

Comments:

This enzyme from Staphylococcus aureus catalyses the transfer of glycine from a charged tRNA to MurNAc-L-Ala-D-isoglutaminyl-L-Lys-D-Ala-D-Ala-diphosphoundecaprenyl-GlcNAc (lipid II), attaching it to the N6 of the L-lysine at position 3 of the pentapeptide. This is the first step in the synthesis of the pentaglycine interpeptide bridge that is used in S. aureus for the crosslinking of different glycan strands to each other. Four additional glycine residues are subsequently attached by EC 2.3.2.17 (FemA) and EC 2.3.2.18 (FemB).

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (14) [show] [UniProt]

References

  1. Schneider, T., Senn, M. M., Berger-Bachi, B., Tossi, A., Sahl, H. G., Wiedemann, I.
    In vitro assembly of a complete, pentaglycine interpeptide bridge containing cell wall precursor (lipid II-Gly5) of Staphylococcus aureus.
    Mol. Microbiol. 53: 675-685 (2004). [PMID: 15228543]

[EC 2.3.2.16 created 2010]