EC - Peptidyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
Other names:
ribosomal peptidyltransferase
Systematic name:
peptidyl-tRNA:aminoacyl-tRNA N-peptidyltransferase



The enzyme is a ribozyme. Two non-equivlant ribonucleoprotein subunits operate in non-concerted fashion in peptide elongation. The small subunit forms the mRNA-binding machinery and decoding center, the large subunit performs the main ribosomal catalytic function in the peptidyl-transferase center.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0000048
CAS Registry Number: 9059-29-4


  1. Rychlik, I.
    Release of lysine peptides by puromycin from polylysyl-transfer ribonucleic acid in the presence of ribosomes.
    Biochim. Biophys. Acta 114 : 425-427 (1966). [PMID: 5329275]
  2. Rychlik, I., Cerná, J., Chládek, S., Zemlicka, J. and Haladová, Z.
    Substrate specificity of ribosomal peptidyl transferase: 2'(3')-O-aminoacyl nucleosides as acceptors of the peptide chain on the amino acid site.
    J. Mol. Biol. 43 : 13-24 (1969). [PMID: 4897787]
  3. Traut, R.R. and Monro, R.E.
    The puromycin reaction and its relation to protein synthesis.
    J. Mol. Biol. 10 : 63-72 (1964).
  4. Voorhees, R. M., Weixlbaumer, A., Loakes, D., Kelley, A. C., Ramakrishnan, V.
    Insights into substrate stabilization from snapshots of the peptidyl transferase center of the intact 70S ribosome.
    Nat. Struct. Mol. Biol. 16 : 528-533 (2009). [PMID: 19363482]

[EC created 1976, modified 2010]