EC - Acetyl-CoA C-acetyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
acetyl-CoA C-acetyltransferase
Other names:
2-methylacetoacetyl-CoA thiolase
β-acetoacetyl coenzyme A thiolase
acetoacetyl-CoA thiolase
acetyl coenzyme A thiolase
acetyl-CoA acetyltransferase
thiolase II
Systematic name:
acetyl-CoA:acetyl-CoA C-acetyltransferase



The enzyme, found in both eukaryotes and prokaryotes, catalyses the Claisen condensation of an acetyl-CoA and an acyl-CoA (often another acetyl-CoA), leading to the formation of an acyl-CoA that is longer by two carbon atoms. The reaction starts with the acylation of a nucleophilic cysteine at the active site, usually by acetyl-CoA but potentially by a different acyl-CoA, with concomitant release of CoA. In the second step the acyl group is transferred to an acetyl-CoA molecule. Cf. EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , NIST 74 , UM-BBD , UniPathway
Protein domains and families: PROSITE:PDOC00092
Structural data: CSA , EC2PDB
Gene Ontology: GO:0003985
CAS Registry Number: 9027-46-7
UniProtKB/Swiss-Prot: (80) [show] [UniProt]


  1. Lynen, F. and Ochoa, S.
    Enzymes of fatty acid metabolism.
    Biochim. Biophys. Acta 12 : 299-314 (1953). [PMID: 13115439]
  2. Stern, J.R., Drummond, G.I., Coon, M.J. and del Campillo, A.
    Enzymes of ketone body metabolism. I. Purification of an acetoacetate-synthesizing enzyme from ox liver.
    J. Biol. Chem. 235 : 313-317 (1960). [PMID: 13834445]

[EC created 1961, updated 2019]