EC - N-terminal methionine Nα-acetyltransferase NatF

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IntEnz Enzyme Nomenclature


Accepted name:
N-terminal methionine Nα-acetyltransferase NatF
Other name:
NAA60 (gene name)
Systematic name:
acetyl-CoA:N-terminal-Met-Lys/Ser/Val/Leu/Gln/Ile/Tyr/Thr-[transmembrane protein] Met-Nα-acetyltransferase



N-terminal-acetylases (NATs) catalyse the covalent attachment of an acetyl moiety from acetyl-CoA to the free α-amino group at the N-terminus of a protein. This irreversible modification neutralizes the positive charge at the N-terminus, makes the N-terminal residue larger and more hydrophobic, and prevents its removal by hydrolysis. NatF is found only in higher eukaryotes, and is absent from yeast. Unlike other Nat systems the enzyme is located in the Golgi apparatus. It faces the cytosolic side of intracellular membranes, and specifically acetylates transmembrane proteins whose N termini face the cytosol. NatF targets N-terminal L-methionine residues attached to Lys, Ser, Val, Leu, Gln, Ile, Tyr and Thr residues. Formerly EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (13) [show] [UniProt]


  1. Van Damme, P., Hole, K., Pimenta-Marques, A., Helsens, K., Vandekerckhove, J., Martinho, R. G., Gevaert, K., Arnesen, T.
    NatF contributes to an evolutionary shift in protein N-terminal acetylation and is important for normal chromosome segregation.
    PLoS Genet. 7 : e1002169 (2011). [PMID: 21750686]
  2. Aksnes, H., Van Damme, P., Goris, M., Starheim, K. K., Marie, M., Støve, S. I., Hoel, C., Kalvik, T. V., Hole, K., Glomnes, N., Furnes, C., Ljostveit, S., Ziegler, M., Niere, M., Gevaert, K., Arnesen, T.
    An organellar N(alpha)-acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity.
    Cell Rep 10 : 1362-1374 (2015). [PMID: 25732826]

[created 1989 as EC, part-transferred 2016 to EC]