EC - Carboxymethylproline synthase

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IntEnz Enzyme Nomenclature


Accepted name:
carboxymethylproline synthase
Other name:
CarB [ambiguous]
Systematic name:
malonyl-CoA:(S)-1-pyrroline-5-carboxylate malonyltransferase (cyclizing)



The enzyme is involved in the biosynthesis of the carbapenem β-lactam antibiotic (5R)-carbapen-2-em-3-carboxylate in the bacterium Pectobacterium carotovorum.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB


  1. Sleeman, M. C., Schofield, C. J.
    Carboxymethylproline synthase (CarB), an unusual carbon-carbon bond-forming enzyme of the crotonase superfamily involved in carbapenem biosynthesis.
    J. Biol. Chem. 279 : 6730-6736 (2004). [PMID: 14625287]
  2. Gerratana, B., Arnett, S. O., Stapon, A., Townsend, C. A.
    Carboxymethylproline synthase from Pectobacterium carotorova: a multifaceted member of the crotonase superfamily.
    Biochemistry 43 : 15936-15945 (2004). [PMID: 15595850]
  3. Sorensen, J. L., Sleeman, M. C., Schofield, C. J.
    Synthesis of deuterium labelled L- and D-glutamate semialdehydes and their evaluation as substrates for carboxymethylproline synthase (CarB)--implications for carbapenem biosynthesis.
    Chem. Commun. (Camb.) 1155-1157 (2005). [PMID: 15726176]
  4. Sleeman, M. C., Sorensen, J. L., Batchelar, E. T., McDonough, M. A., Schofield, C. J.
    Structural and mechanistic studies on carboxymethylproline synthase (CarB), a unique member of the crotonase superfamily catalyzing the first step in carbapenem biosynthesis.
    J. Biol. Chem. 280 : 34956-34965 (2005). [PMID: 16096274]
  5. Batchelar, E. T., Hamed, R. B., Ducho, C., Claridge, T. D., Edelmann, M. J., Kessler, B., Schofield, C. J.
    Thioester hydrolysis and C-C bond formation by carboxymethylproline synthase from the crotonase superfamily.
    Angew. Chem. Int. Ed. Engl. 47 : 9322-9325 (2008). [PMID: 18972478]
  6. Hamed, R. B., Gomez-Castellanos, J. R., Thalhammer, A., Harding, D., Ducho, C., Claridge, T. D., Schofield, C. J.
    Stereoselective C-C bond formation catalysed by engineered carboxymethylproline synthases.
    Nat Chem 3 : 365-371 (2011). [PMID: 21505494]

[EC created 2013]