EC 2 - Transferases
EC 2.3 - Acyltransferases
EC 2.3.1 - Transferring groups other than amino-acyl groups
EC 2.3.1.191 - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
IntEnz view
ENZYME view
IntEnz Enzyme Nomenclature
EC 2.3.1.191
Names
Accepted name:
UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase
Other
names:
UDP-3-O-acyl-glucosamine N-acyltransferase
LpxD
acetyltransferase LpxD
acyl-ACP:UDP-3-O-(3-hydroxyacyl)-GlcN N-acyltransferase
firA
UDP-3-O-[3-hydroxymyristoyl]-D-glucosamine N-acyltransferase
UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase
(3R)-3-hydroxymyristoyl-[acyl-carrier protein]:UDP-3-O-[(3R)-hydroxymyristoyl]-α-D-glucosamine N-acyltransferase
LpxD
acetyltransferase LpxD
acyl-ACP:UDP-3-O-(3-hydroxyacyl)-GlcN N-acyltransferase
firA
UDP-3-O-[3-hydroxymyristoyl]-D-glucosamine N-acyltransferase
UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase
(3R)-3-hydroxymyristoyl-[acyl-carrier protein]:UDP-3-O-[(3R)-hydroxymyristoyl]-α-D-glucosamine N-acyltransferase
Systematic name:
(3R)-3-hydroxyacyl-[acyl-carrier protein]:UDP-3-O-[(3R)-hydroxyacyl]-α-D-glucosamine N-acyltransferase
Reaction
- a (3R)-3-hydroxyacyl-acyl-carrier-protein + a UDP-3-O-[(3R)-hydroxyacyl]-alpha-D-glucosamine = a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-glucosamine + a holo-acyl-carrier-protein
Comments:
The enzyme catalyses a step of lipid A biosynthesis. LpxD from Escherichia coli prefers (3R)-3-hydroxytetradecanoyl-[acyl-carrier protein] [3], but it does not an absolute specificity for 14-carbon hydroxy fatty acids, as it can transfer other fatty acids, including odd-chain fatty acids, if they are available to the organism [5].
Links to other databases
Gene Ontology:
GO:0103118
References
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Crystal structure and acyl chain selectivity of Escherichia coli LpxD, the N-acyltransferase of lipid A biosynthesis.Biochemistry 48 : 8672-8683 (2009). [PMID: 19655786]
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Structure and reactivity of LpxD, the N-acyltransferase of lipid A biosynthesis.Proc. Natl. Acad. Sci. USA 104 : 4321-4326 (2007). [PMID: 17360522]
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Steady-state kinetics and mechanism of LpxD, the N-acyltransferase of lipid A biosynthesis.Biochemistry 47 : 5290-5302 (2008). [PMID: 18422345]
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The firA gene of Escherichia coli encodes UDP-3-O-(R-3-hydroxymyristoyl)-glucosamine N-acyltransferase. The third step of endotoxin biosynthesis.J. Biol. Chem. 268 : 19866-19874 (1993). [PMID: 8366125]
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Acyl chain specificity of the acyltransferases LpxA and LpxD and substrate availability contribute to lipid A fatty acid heterogeneity in Porphyromonas gingivalis.J. Bacteriol. 190 : 4549-4558 (2008). [PMID: 18456814]
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Structure determination of LpxD from the lipopolysaccharide-synthesis pathway of Acinetobacter baumannii.Acta Crystallogr Sect F Struct Biol Cryst Commun 69 : 6-9 (2013). [PMID: 23295477]
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Discovery of dual-activity small-molecule ligands of Pseudomonas aeruginosa LpxA and LpxD using SPR and X-ray crystallography.Sci Rep 9 : 15450 (2019). [PMID: 31664082]
[EC 2.3.1.191 created 2010]