EC - Acetyl-CoA C-acyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
acetyl-CoA C-acyltransferase
Other names:
3-ketoacyl CoA thiolase
3-ketoacyl coenzyme A thiolase
3-ketoacyl thiolase
3-ketoacyl-CoA thiolase
3-oxoacyl-CoA thiolase
3-oxoacyl-coenzyme A thiolase
6-oxoacyl-CoA thiolase
β-ketoacyl coenzyme A thiolase
β-ketoacyl-CoA thiolase
β-ketoadipyl coenzyme A thiolase
β-ketoadipyl-CoA thiolase
acetoacetyl-CoA β-ketothiolase
acetyl-CoA acyltransferase
ketoacyl-CoA acyltransferase
ketoacyl-coenzyme A thiolase
long-chain 3-oxoacyl-CoA thiolase
oxoacyl-coenzyme A thiolase
pro-3-ketoacyl-CoA thiolase
thiolase I
2-methylacetoacetyl-CoA thiolase
Systematic name:
acyl-CoA:acetyl-CoA C-acyltransferase



The enzyme, found in both eukaryotes and in prokaryotes, is involved in degradation pathways such as fatty acid β-oxidation. The enzyme acts on 3-oxoacyl-CoAs to produce acetyl-CoA and an acyl-CoA shortened by two carbon atoms. The reaction starts with the acylation of a nucleophilic cysteine at the active site by a 3-oxoacyl-CoA, with the concomitant release of acetyl-CoA. In the second step the acyl group is transferred to CoA. Most enzymes have a broad substrate range for the 3-oxoacyl-CoA. cf. EC

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00092
Structural data: CSA , EC2PDB
Gene Ontology: GO:0003988
CAS Registry Number: 9029-97-4
UniProtKB/Swiss-Prot: (242) [show] [UniProt]


  1. Beinert, H., Bock, R.M., Goldman, D.S., Green, D.E., Mahler, H.R., Mii, S., Stansly, P.G. and Wakil, S.J.
    A synthesis of dl-cortisone acetate.
    J. Am. Chem. Soc. 75 : 4111-4112 (1953).
  2. Goldman, D.S.
    Studies on the fatty acid oxidizing system of animal tissue. VII. The β-ketoacyl coenzyme A cleavage enzyme.
    J. Biol. Chem. 208 : 345-357 (1954). [PMID: 13174544]
  3. Stern, J.R., Coon, M.J. and del Campillo, A.
    Enzymatic breakdown and synthesis of acetoacetate.
    Nature 171 : 28-30 (1953).

[EC created 1961]