EC 2.1.2.2 - Phosphoribosylglycinamide formyltransferase 1

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IntEnz Enzyme Nomenclature
EC 2.1.2.2

Names

Accepted name:
phosphoribosylglycinamide formyltransferase 1
Other names:
2-amino-N-ribosylacetamide 5'-phosphate transformylase
5,10-methenyltetrahydrofolate:2-amino-N-ribosylacetamide ribonucleotide transformylase
GAR TFase
GAR formyltransferase
GAR transformylase
glycinamide ribonucleotide transformylase
GART
5'-phosphoribosylglycinamide transformylase
Systematic name:
10-formyltetrahydrofolate:5'-phosphoribosylglycinamide N-formyltransferase

Reaction

Comments:

Two enzymes are known to catalyse the third step in de novo purine biosynthesis. This enzyme utilizes 10-formyltetrahydrofolate as the formyl donor, while the other enzyme, EC 6.3.1.21, phosphoribosylglycinamide formyltransferase 2, utilizes formate. In vertebrates this activity is catalysed by a trifunctional enzyme that also catalyses the activities of EC 6.3.4.13, phosphoribosylamine—glycine ligase and EC 6.3.3.1, phosphoribosylformylglycinamidine cyclo-ligase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00319
Structural data: CSA , EC2PDB
Gene Ontology: GO:0004644
CAS Registry Number: 9032-02-4
UniProtKB/Swiss-Prot: (31) [show] [UniProt]

References

  1. Hartman, S.C. and Buchanan, J.M.
    Biosynthesis of the purines. XXVI. The identification of the formyl donors of the transformylation reaction.
    J. Biol. Chem. 234 : 1812-1816 (1959). [PMID: 13672969]
  2. Smith, G.K., Benkovic, P.A. and Benkovic, S.J.
    L(-)-10-Formyltetrahydrofolate is the cofactor for glycinamide ribonucleotide transformylase from chicken liver.
    Biochemistry 20 : 4034-4036 (1981). [PMID: 7284307]
  3. Warren, L. and Buchanan, J.M.
    Biosynthesis of the purines. XIX. 2-Amino-N-ribosylacetamide 5'-phosphate (glycinamide ribotide) transformylase.
    J. Biol. Chem. 229 : 613-626 (1957). [PMID: 13502326]
  4. Schild, D., Brake, A. J., Kiefer, M. C., Young, D., Barr, P. J.
    Cloning of three human multifunctional de novo purine biosynthetic genes by functional complementation of yeast mutations.
    Proc. Natl. Acad. Sci. U.S.A. 87 : 2916-2920 (1990). [PMID: 2183217]
  5. Zhang, Y., Desharnais, J., Greasley, S. E., Beardsley, G. P., Boger, D. L., Wilson, I. A.
    Crystal structures of human GAR Tfase at low and high pH and with substrate beta-GAR.
    Biochemistry 41 : 14206-14215 (2002). [PMID: 12450384]

[EC 2.1.2.2 created 1961, modified 2000, modified 2021]