EC - Tetrahydromethanopterin S-methyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
tetrahydromethanopterin S-methyltransferase
Other names:
tetrahydromethanopterin methyltransferase
N5-methyltetrahydromethanopterin--coenzyme M methyltransferase
mtrA-H (gene names)
cmtA (gene name)
5-methyl-5,6,7,8-tetrahydromethanopterin:2-mercaptoethanesulfonate 2-methyltransferase
Systematic name:
5-methyl-5,6,7,8-tetrahydromethanopterin:coenzyme-M 2-methyltransferase (Na+-transporting)



Involved in the formation of methane from CO2 in methanogenic archaea. The reaction involves the export of one or two sodium ions. The enzyme from the archaeon Methanobacterium thermoautotrophicum is a membrane-associated multienzyme complex composed of eight different subunits, and contains a 5'-hydroxybenzimidazolyl-cobamide prosthetic group, to which the methyl group is attached during the transfer. A soluble enzyme that is induced by the presence of CO has been reported as well.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , DIAGRAM , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UM-BBD , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0030269
CAS Registry Number: 103406-60-6
UniProtKB/Swiss-Prot: (93) [show] [UniProt]


  1. Sauer, F.D.
    Tetrahydromethanopterin methyltransferase, a component of the methane synthesizing complex of Methanobacterium thermoautotrophicum.
    Biochem. Biophys. Res. Commun. 136: 542-547 (1986). [PMID: 3085670]
  2. Gartner, P., Ecker, A., Fischer, R., Linder, D., Fuchs, G., Thauer, R. K.
    Purification and properties of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase from Methanobacterium thermoautotrophicum.
    Eur. J. Biochem. 213: 537-545 (1993). [PMID: 8477726]
  3. Weiss, D. S., Gartner, P., Thauer, R. K.
    The energetics and sodium-ion dependence of N5-methyltetrahydromethanopterin:coenzyme M methyltransferase studied with cob(I)alamin as methyl acceptor and methylcob(III)alamin as methyl donor.
    Eur. J. Biochem. 226: 799-809 (1994). [PMID: 7813469]
  4. Harms, U., Weiss, D. S., Gartner, P., Linder, D., Thauer, R. K.
    The energy conserving N5-methyltetrahydromethanopterin:coenzyme M methyltransferase complex from Methanobacterium thermoautotrophicum is composed of eight different subunits.
    Eur. J. Biochem. 228: 640-648 (1995). [PMID: 7737157]
  5. Gottschalk, G., Thauer, R. K.
    The Na(+)-translocating methyltransferase complex from methanogenic archaea.
    Biochim. Biophys. Acta 1505: 28-36 (2001). [PMID: 11248186]
  6. Vepachedu, V. R., Ferry, J. G.
    Role of the fused corrinoid/methyl transfer protein CmtA during CO-dependent growth of Methanosarcina acetivorans.
    J. Bacteriol. 194: 4161-4168 (2012). [PMID: 22636775]

[EC created 1989, modified 2000, modified 2017]