IntEnz

EC 2.1.1.359 - [histone H3]-lysine³⁶ N-trimethyltransferase

IntEnz Enzyme Nomenclature
EC 2.1.1.359

Names

Reaction

• 60324 [IUBMB]
  L-lysyl³⁶-[histone H3]

  L-lysyl(36)-[histone H3]

  GENERIC:9785

  Is ROOT: no
  ROOT compound: GENERIC:9784

  Number of residues: 1

  L-lysine residue L-lysine residue Name origin: UniProt - CHECKED (C) CHEBI:29969 Formula: C6H13N2O Charge: 1 Position: 36 ChEBI compound status: CHECKED (C)

  +

  3

  S-adenosyl-L-methionine

  S-adenosyl-L-methionine

  Name origin: UniProt - CHECKED (C)

  CHEBI:59789

  Formula: C15H23N6O5S
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  =

  3

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  N⁶,N⁶,N⁶-trimethyl-L-lysyl³⁶-[histone H3]

  N(6),N(6),N(6)-trimethyl-L-lysyl(36)-[histone H3]

  GENERIC:15536

  Is ROOT: no
  ROOT compound: GENERIC:9784

  Number of residues: 1

  N6,N6,N6-trimethyl-L-lysine residue N(6),N(6),N(6)-trimethyl-L-lysine residue Name origin: UniProt - CHECKED (C) CHEBI:61961 Formula: C9H19N2O Charge: 1 Position: 36 ChEBI compound status: CHECKED (C)

  +

  3

  S-adenosyl-L-homocysteine

  S-adenosyl-L-homocysteine

  Name origin: UniProt - CHECKED (C)

  CHEBI:57856

  Formula: C14H20N6O5S
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

Comments:

The enzyme, characterized from yeast, catalyses the successive methylation of lysine³⁶ of histone H3 (H3K36), forming the trimethylated form. These modifications influence the binding of chromatin-associated proteins. The enzyme couples the methylation reactions with transcriptional elongation through an interaction with the large subunit of RNA polymerase II. In mammals this activity is catalysed by two different enzymes, EC 2.1.1.357, [histone H3]-lysine³⁶ N-dimethyltransferase and EC 2.1.1.358, [histone H3]-dimethyl-L-lysine³⁶ N-methyltransferase.

Links to other databases

References

1. Strahl, B. D., Grant, P. A., Briggs, S. D., Sun, Z. W., Bone, J. R., Caldwell, J. A., Mollah, S., Cook, R. G., Shabanowitz, J., Hunt, D. F., Allis, C. D.
   Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression.
   Mol. Cell. Biol. 22: 1298-1306 (2002). [PMID: 11839797]
2. Landry, J., Sutton, A., Hesman, T., Min, J., Xu, R. M., Johnston, M., Sternglanz, R.
   Set2-catalyzed methylation of histone H3 represses basal expression of GAL4 in Saccharomyces cerevisiae.
   Mol. Cell. Biol. 23: 5972-5978 (2003). [PMID: 12917322]
3. Morris, S. A., Shibata, Y., Noma, K., Tsukamoto, Y., Warren, E., Temple, B., Grewal, S. I., Strahl, B. D.
   Histone H3 K36 methylation is associated with transcription elongation in Schizosaccharomyces pombe.
   Eukaryotic Cell 4: 1446-1454 (2005). [PMID: 16087749]
4. Lin, L. J., Minard, L. V., Johnston, G. C., Singer, R. A., Schultz, M. C.
   Asf1 can promote trimethylation of H3 K36 by Set2.
   Mol. Cell. Biol. 30: 1116-1129 (2010). [PMID: 20048053]

[EC 2.1.1.359 created 2019 created 1976 as EC 2.1.1.43, modified 1982, modified 1983, part transferred 2019 to EC 2.1.1.359]