EC - TRNA (uracil54-C5)-methyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
tRNA (uracil54-C5)-methyltransferase
Other names:
ribothymidyl synthase
tRNA uracil 5-methyltransferase
transfer RNA uracil 5-methyltransferase
transfer RNA uracil methylase
transfer RNA uracil54 5-methyltransferase
transfer RNA uracil54 methylase
tRNA uracil54 5-methyltransferase
5-methyluridine54 tRNA methyltransferase
Systematic name:
S-adenosyl-L-methionine:tRNA (uracil54-C5)-methyltransferase



Unlike this enzyme, EC (methylenetetrahydrofolate—tRNA-(uracil54-C5)-methyltransferase (FADH2-oxidizing)), uses 5,10-methylenetetrahydrofolate and FADH2 to supply the atoms for methylation of U54 [4].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00945
Structural data: CSA , EC2PDB
Gene Ontology: GO:0030697
CAS Registry Number: 37257-02-6
UniProtKB/Swiss-Prot: (193) [show] [UniProt]


  1. Björk, G.R. and Svensson, I.
    Studies on microbial RNA. Fractionation of tRNA methylases from Saccharomyces cerevisiae.
    Eur. J. Biochem. 9: 207-215 (1969). [PMID: 4896260]
  2. Greenberg, R. and Dudock, B.
    Isolation and characterization of m5U-methyltransferase from Escherichia coli.
    J. Biol. Chem. 255: 8296-8302 (1980). [PMID: 6997293]
  3. Hurwitz, J., Gold, M. and Anders, M.
    The enzymatic methylation of ribonucleic acid and deoxyribonucleic acid. 3. Purification of soluble ribonucleic acid-methylating enzymes.
    J. Biol. Chem. 239: 3462-3473 (1964). [PMID: 14245404]
  4. Delk, A.S., Nagle, D.P., Jr. and Rabinowitz, J.C.
    Methylenetetrahydrofolate-dependent biosynthesis of ribothymidine in transfer RNA of Streptococcus faecalis. Evidence for reduction of the 1-carbon unit by FADH2.
    J. Biol. Chem. 255: 4387-4390 (1980). [PMID: 6768721]
  5. Kealey, J. T., Gu, X. and Santi, D. V.
    Enzymatic mechanism of tRNA (m5U54)methyltransferase.
    Biochimie 76: 1133-1142 (1994). [PMID: 7748948]
  6. Gu, X., Ivanetich, K.M. and Santi, D.V.
    Recognition of the T-arm of tRNA by tRNA (m5U54)-methyltransferase is not sequence specific.
    Biochemistry 35: 11652-11659 (1996). [PMID: 8794745]
  7. Becker, H. F., Motorin, Y., Sissler, M., Florentz, C., Grosjean, H.
    Major identity determinants for enzymatic formation of ribothymidine and pseudouridine in the TΨ-loop of yeast tRNAs.
    J. Mol. Biol. 274: 505-518 (1997). [PMID: 9417931]
  8. Walbott, H., Leulliot, N., Grosjean, H., Golinelli-Pimpaneau, B.
    The crystal structure of Pyrococcus abyssi tRNA (uracil-54, C5)-methyltransferase provides insights into its tRNA specificity.
    Nucleic Acids Res. 36: 4929-4940 (2008). [PMID: 18653523]

[EC created 1972, modified 2011]