EC - TRNA (guanosine18-2'-O)-methyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
tRNA (guanosine18-2'-O)-methyltransferase
Other names:
tRNA (Gm18) methyltransferase
tRNA (Gm18) 2'-O-methyltransferase
Systematic name:
S-adenosyl-L-methionine:tRNA (guanosine18-2'-O)-methyltransferase



The enzyme catalyses the methylation of guanosine18 in tRNA.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0009020
CAS Registry Number: 37257-01-5


  1. Gefter, M.L.
    The in vitro synthesis of 2'-O-methylguanosine and 2-methylthio 6N (γ,γ-dimethylallyl) adenosine in transfer RNA of Escherichia coli.
    Biochem. Biophys. Res. Commun. 36 : 435-441 (1969). [PMID: 4898378]
  2. Kumagai, I., Watanabe, K. and Oshima, T.
    Thermally induced biosynthesis of 2'-O-methylguanosine in tRNA from an extreme thermophile, Thermus thermophilus HB27.
    Proc. Natl. Acad. Sci. USA 77 : 1922-1926 (1980). [PMID: 6990416]
  3. Hori, H., Yamazaki, N., Matsumoto, T., Watanabe, Y., Ueda, T., Nishikawa, K., Kumagai, I. and Watanabe, K.
    Substrate recognition of tRNA (guanosine-2'-)-methyltransferase from Thermus thermophilus HB27.
    J. Biol. Chem. 273 : 25721-25727 (1998). [PMID: 9748240]
  4. Pleshe, E., Truesdell, J., Batey, R. T.
    Structure of a class II TrmH tRNA-modifying enzyme from Aquifex aeolicus.
    Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 61 : 722-728 (2005). [PMID: 16511140]
  5. Ochi, A., Makabe, K., Kuwajima, K., Hori, H.
    Flexible recognition of the tRNA G18 methylation target site by TrmH methyltransferase through first binding and induced fit processes.
    J. Biol. Chem. 285 : 9018-9029 (2010). [PMID: 20053984]

[EC created 1972, modified 2005, modified 2011]