EC - Erythromycin 3''-O-methyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
erythromycin 3''-O-methyltransferase
Other name:
Systematic name:
S-adenosyl-L-methionine:erythromycin C 3''-O-methyltransferase



The enzyme methylates the 3 position of the mycarosyl moiety of erythromycin C, forming the most active form of the antibiotic, erythromycin A. It can also methylate the precursor erythromycin D, forming erythromycin B, which is then converted to erythromycin A by EC, erythromycin 12 hydroxylase.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102307 , GO:0102308


  1. Paulus, T. J., Tuan, J. S., Luebke, V. E., Maine, G. T., DeWitt, J. P., Katz, L.
    Mutation and cloning of eryG, the structural gene for erythromycin O-methyltransferase from Saccharopolyspora erythraea, and expression of eryG in Escherichia coli.
    J. Bacteriol. 172 : 2541-2546 (1990). [PMID: 2185226]
  2. Summers, R. G., Donadio, S., Staver, M. J., Wendt-Pienkowski, E., Hutchinson, C. R., Katz, L.
    Sequencing and mutagenesis of genes from the erythromycin biosynthetic gene cluster of Saccharopolyspora erythraea that are involved in L-mycarose and D-desosamine production.
    Microbiology (Reading, Engl.) 143 : 3251-3262 (1997). [PMID: 9353926]

[EC created 2012]