EC 2.1.1.246 - [methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase

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IntEnz Enzyme Nomenclature
EC 2.1.1.246

Names

Accepted name:
[methyl-Co(III) methanol-specific corrinoid protein]:coenzyme M methyltransferase
Other names:
methyltransferase 2 [ambiguous]
mtaA (gene name)
Systematic name:
methylated methanol-specific corrinoid protein:coenzyme M methyltransferase

Reaction

Comments:

The enzyme, which is involved in methanogenesis from methanol, catalyses the transfer of a methyl group from a corrinoid protein (see EC 2.1.1.90, methanol—corrinoid protein Co-methyltransferase), where it is bound to the cobalt cofactor, to coenzyme M, forming the substrate for EC 2.8.4.1, coenzyme-B sulfoethylthiotransferase, the enzyme that catalyses the final step in methanogenesis. Free methylcob(I)alamin can substitute for the corrinoid protein in vitro [5].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
Gene Ontology: GO:1990088
UniProtKB/Swiss-Prot:

References

  1. LeClerc, G. M., Grahame, D. A.
    Methylcobamide:coenzyme M methyltransferase isozymes from Methanosarcina barkeri. Physicochemical characterization, cloning, sequence analysis, and heterologous gene expression.
    J. Biol. Chem. 271: 18725-18731 (1996). [PMID: 8702528]
  2. Harms, U., Thauer, R. K.
    Methylcobalamin: coenzyme M methyltransferase isoenzymes MtaA and MtbA from Methanosarcina barkeri. Cloning, sequencing and differential transcription of the encoding genes, and functional overexpression of the mtaA gene in Escherichia coli.
    Eur. J. Biochem. 235: 653-659 (1996). [PMID: 8654414]
  3. Sauer, K., Thauer, R. K.
    Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Zinc dependence and thermodynamics of the methanol:cob(I)alamin methyltransferase reaction.
    Eur. J. Biochem. 249: 280-285 (1997). [PMID: 9363780]
  4. Sauer, K., Harms, U., Thauer, R. K.
    Methanol:coenzyme M methyltransferase from Methanosarcina barkeri. Purification, properties and encoding genes of the corrinoid protein MT1.
    Eur. J. Biochem. 243: 670-677 (1997). [PMID: 9057830]
  5. Sauer, K., Thauer, R. K.
    Methanol:coenzyme M methyltransferase from Methanosarcina barkeri -- substitution of the corrinoid harbouring subunit MtaC by free cob(I)alamin.
    Eur. J. Biochem. 261: 674-681 (1999). [PMID: 10215883]

[EC 2.1.1.246 created 2012]