IntEnz

EC 2.1.1.202 - Multisite-specific tRNA:(cytosine-C⁵)-methyltransferase

IntEnz Enzyme Nomenclature
EC 2.1.1.202

Names

Reactions

• 42940 [IUBMB]
  cytidine³⁴ in tRNA precursor

  cytidine(34) in tRNA precursor

  GENERIC:10291

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  CMP residue CMP residue Name origin: UniProt - CHECKED (C) CHEBI:82748 Formula: C9H11N3O7P Charge: -1 Position: 34 ChEBI compound status: CHECKED (C)

  +

  S-adenosyl-L-methionine

  S-adenosyl-L-methionine

  Name origin: UniProt - CHECKED (C)

  CHEBI:59789

  Formula: C15H23N6O5S
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  =

  5-methylcytidine³⁴ in tRNA precursor

  5-methylcytidine(34) in tRNA precursor

  GENERIC:10295

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  5-methylcytidine 5'-phosphate residue 5-methylcytidine 5'-phosphate residue Name origin: UniProt - CHECKED (C) CHEBI:74483 Formula: C10H13N3O7P Charge: -1 Position: 34 ChEBI compound status: CHECKED (C)

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  S-adenosyl-L-homocysteine

  S-adenosyl-L-homocysteine

  Name origin: UniProt - CHECKED (C)

  CHEBI:57856

  Formula: C14H20N6O5S
  Charge: 0

  ChEBI compound status: CHECKED (C)

  
• 42944 [IUBMB]
  cytidine⁴⁰ in tRNA precursor

  cytidine(40) in tRNA precursor

  GENERIC:10292

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  CMP residue CMP residue Name origin: UniProt - CHECKED (C) CHEBI:82748 Formula: C9H11N3O7P Charge: -1 Position: 40 ChEBI compound status: CHECKED (C)

  +

  S-adenosyl-L-methionine

  S-adenosyl-L-methionine

  Name origin: UniProt - CHECKED (C)

  CHEBI:59789

  Formula: C15H23N6O5S
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  =

  5-methylcytidine⁴⁰ in tRNA precursor

  5-methylcytidine(40) in tRNA precursor

  GENERIC:10296

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  5-methylcytidine 5'-phosphate residue 5-methylcytidine 5'-phosphate residue Name origin: UniProt - CHECKED (C) CHEBI:74483 Formula: C10H13N3O7P Charge: -1 Position: 40 ChEBI compound status: CHECKED (C)

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  S-adenosyl-L-homocysteine

  S-adenosyl-L-homocysteine

  Name origin: UniProt - CHECKED (C)

  CHEBI:57856

  Formula: C14H20N6O5S
  Charge: 0

  ChEBI compound status: CHECKED (C)

  
• 42948 [IUBMB]
  cytidine⁴⁸ in tRNA

  cytidine(48) in tRNA

  GENERIC:10293

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  CMP residue CMP residue Name origin: UniProt - CHECKED (C) CHEBI:82748 Formula: C9H11N3O7P Charge: -1 Position: 48 ChEBI compound status: CHECKED (C)

  +

  S-adenosyl-L-methionine

  S-adenosyl-L-methionine

  Name origin: UniProt - CHECKED (C)

  CHEBI:59789

  Formula: C15H23N6O5S
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  =

  5-methylcytidine⁴⁸ in tRNA

  5-methylcytidine(48) in tRNA

  GENERIC:10297

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  5-methylcytidine 5'-phosphate residue 5-methylcytidine 5'-phosphate residue Name origin: UniProt - CHECKED (C) CHEBI:74483 Formula: C10H13N3O7P Charge: -1 Position: 48 ChEBI compound status: CHECKED (C)

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  S-adenosyl-L-homocysteine

  S-adenosyl-L-homocysteine

  Name origin: UniProt - CHECKED (C)

  CHEBI:57856

  Formula: C14H20N6O5S
  Charge: 0

  ChEBI compound status: CHECKED (C)

  
• 42952 [IUBMB]
  cytidine⁴⁹ in tRNA

  cytidine(49) in tRNA

  GENERIC:10294

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  CMP residue CMP residue Name origin: UniProt - CHECKED (C) CHEBI:82748 Formula: C9H11N3O7P Charge: -1 Position: 49 ChEBI compound status: CHECKED (C)

  +

  S-adenosyl-L-methionine

  S-adenosyl-L-methionine

  Name origin: UniProt - CHECKED (C)

  CHEBI:59789

  Formula: C15H23N6O5S
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  =

  5-methylcytidine⁴⁹ in tRNA

  5-methylcytidine(49) in tRNA

  GENERIC:10385

  Is ROOT: no
  ROOT compound: GENERIC:9579

  Number of residues: 1

  5-methylcytidine 5'-phosphate residue 5-methylcytidine 5'-phosphate residue Name origin: UniProt - CHECKED (C) CHEBI:74483 Formula: C10H13N3O7P Charge: -1 Position: 49 ChEBI compound status: CHECKED (C)

  +

  H⁺

  H(+)

  Name origin: UniProt - CHECKED (C)

  CHEBI:15378

  Formula: H
  Charge: 1

  ChEBI compound status: CHECKED (C)

  

  +

  S-adenosyl-L-homocysteine

  S-adenosyl-L-homocysteine

  Name origin: UniProt - CHECKED (C)

  CHEBI:57856

  Formula: C14H20N6O5S
  Charge: 0

  ChEBI compound status: CHECKED (C)

  

Comments:

The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA. The incidence of modification depends on the cytosine position in tRNA. At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNA^Leu(CUA) and tRNA^Phe(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine⁴⁸ or 5-methylcytosine⁴⁹, but never both in the same tRNA molecule [1]. The formation of 5-methylcytosine³⁴ and 5-methylcytosine⁴⁰ is a strictly intron-dependent process, whereas the formation of 5-methylcytosine⁴⁸ and 5-methylcytosine⁴⁹ is an intron-independent process [2,3].

Links to other databases

References

1. Motorin, Y., Grosjean, H.
   Multisite-specific tRNA:m⁵C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme.
   RNA 5: 1105-1118 (1999). [PMID: 10445884]
2. Jiang, H. Q., Motorin, Y., Jin, Y. X., Grosjean, H.
   Pleiotropic effects of intron removal on base modification pattern of yeast tRNA^Phe: an in vitro study.
   Nucleic Acids Res. 25: 2694-2701 (1997). [PMID: 9207014]
3. Strobel, M. C., Abelson, J.
   Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.
   Mol. Cell. Biol. 6: 2663-2673 (1986). [PMID: 3537724]
4. Walbott, H., Husson, C., Auxilien, S., Golinelli-Pimpaneau, B.
   Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m⁵C methyltransferase.
   RNA 13: 967-973 (2007). [PMID: 17475914]

[EC 2.1.1.202 created 1976 as EC 2.1.1.29, part-transferred 2011 to EC 2.1.1.202]