EC 2.1.1.202 - Multisite-specific tRNA:(cytosine-C5)-methyltransferase
IntEnz Enzyme Nomenclature
EC 2.1.1.202
Names
TRM4
Reactions
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42940 [IUBMB]cytidine34 in tRNA precursorGENERIC:10291Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1S-adenosyl-L-methionineName origin: UniProt - CHECKED (C)Formula: C15H23N6O5S
Charge: 1ChEBI compound status: CHECKED (C)=5-methylcytidine34 in tRNA precursorGENERIC:10295Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1H+Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C) -
42944 [IUBMB]cytidine40 in tRNA precursorGENERIC:10292Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1S-adenosyl-L-methionineName origin: UniProt - CHECKED (C)Formula: C15H23N6O5S
Charge: 1ChEBI compound status: CHECKED (C)=5-methylcytidine40 in tRNA precursorGENERIC:10296Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1H+Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C) -
42948 [IUBMB]cytidine48 in tRNAGENERIC:10293Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1S-adenosyl-L-methionineName origin: UniProt - CHECKED (C)Formula: C15H23N6O5S
Charge: 1ChEBI compound status: CHECKED (C)=5-methylcytidine48 in tRNAGENERIC:10297Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1H+Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C) -
42952 [IUBMB]cytidine49 in tRNAGENERIC:10294Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1S-adenosyl-L-methionineName origin: UniProt - CHECKED (C)Formula: C15H23N6O5S
Charge: 1ChEBI compound status: CHECKED (C)=5-methylcytidine49 in tRNAGENERIC:10385Is ROOT: no
ROOT compound: GENERIC:9579Number of residues: 1H+Name origin: UniProt - CHECKED (C)Formula: H
Charge: 1ChEBI compound status: CHECKED (C)
Comments:
The enzyme from Saccharomyces cerevisiae is responsible for complete 5-methylcytosine methylations of yeast tRNA. The incidence of modification depends on the cytosine position in tRNA. At positions 34 and 40, 5-methylcytosine is found only in two yeast tRNAs (tRNALeu(CUA) and tRNAPhe(GAA), respectively), whereas most other elongator yeast tRNAs bear either 5-methylcytosine48 or 5-methylcytosine49, but never both in the same tRNA molecule [1]. The formation of 5-methylcytosine34 and 5-methylcytosine40 is a strictly intron-dependent process, whereas the formation of 5-methylcytosine48 and 5-methylcytosine49 is an intron-independent process [2,3].
Links to other databases
References
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Multisite-specific tRNA:m5C-methyltransferase (Trm4) in yeast Saccharomyces cerevisiae: identification of the gene and substrate specificity of the enzyme.RNA 5 : 1105-1118 (1999). [PMID: 10445884]
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Pleiotropic effects of intron removal on base modification pattern of yeast tRNAPhe: an in vitro study.Nucleic Acids Res. 25 : 2694-2701 (1997). [PMID: 9207014]
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Effect of intron mutations on processing and function of Saccharomyces cerevisiae SUP53 tRNA in vitro and in vivo.Mol. Cell. Biol. 6 : 2663-2673 (1986). [PMID: 3537724]
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Cysteine of sequence motif VI is essential for nucleophilic catalysis by yeast tRNA m5C methyltransferase.RNA 13 : 967-973 (2007). [PMID: 17475914]
[EC 2.1.1.202 created 1976 as EC 2.1.1.29, part-transferred 2011 to EC 2.1.1.202]