EC - 23S rRNA (uracil1939-C5)-methyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
23S rRNA (uracil1939-C5)-methyltransferase
Other names:
RNA uridine methyltransferase A
Systematic name:
S-adenosyl-L-methionine:23S rRNA (uracil1939-C5)-methyltransferase



The enzyme specifically methylates uracil1939 at C5 in 23S rRNA [1]. The enzyme contains an [4Fe-4S] cluster coordinated by four conserved cysteine residues [2].

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Structural data: CSA , EC2PDB
UniProtKB/Swiss-Prot: (163) [show] [UniProt]


  1. Agarwalla, S., Kealey, J. T., Santi, D. V., Stroud, R. M.
    Characterization of the 23 S ribosomal RNA m5U1939 methyltransferase from Escherichia coli.
    J. Biol. Chem. 277: 8835-8840 (2002). [PMID: 11779873]
  2. Lee, T. T., Agarwalla, S., Stroud, R. M.
    Crystal structure of RumA, an iron-sulfur cluster containing E. coli ribosomal RNA 5-methyluridine methyltransferase.
    Structure 12: 397-407 (2004). [PMID: 15016356]
  3. Madsen, C. T., Mengel-Jørgensen, J., Kirpekar, F., Douthwaite, S.
    Identifying the methyltransferases for m5U747 and m5U1939 in 23S rRNA using MALDI mass spectrometry.
    Nucleic Acids Res. 31: 4738-4746 (2003). [PMID: 12907714]
  4. Persaud, C., Lu, Y., Vila-Sanjurjo, A., Campbell, J. L., Finley, J., O'Connor, M.
    Mutagenesis of the modified bases, m5U1939 and ψ2504, in Escherichia coli 23S rRNA.
    Biochem. Biophys. Res. Commun. 392: 223-227 (2010). [PMID: 20067766]
  5. Agarwalla, S., Stroud, R. M., Gaffney, B. J.
    Redox reactions of the iron-sulfur cluster in a ribosomal RNA methyltransferase, RumA: optical and EPR studies.
    J. Biol. Chem. 279: 34123-34129 (2004). [PMID: 15181002]
  6. Lee, T. T., Agarwalla, S., Stroud, R. M.
    A unique RNA Fold in the RumA-RNA-cofactor ternary complex contributes to substrate selectivity and enzymatic function.
    Cell 120: 599-611 (2005). [PMID: 15766524]

[EC created 2010]