EC - Demethylmenaquinone methyltransferase

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IntEnz Enzyme Nomenclature


Accepted name:
demethylmenaquinone methyltransferase
Other names:
S-adenosyl-L-methione—DMK methyltransferase
demethylmenaquinone C-methylase
2-heptaprenyl-1,4-naphthoquinone methyltransferase
2-demethylmenaquinone methyltransferase
S-adenosyl-L-methione:2-demethylmenaquinone methyltransferase
Systematic name:
S-adenosyl-L-methione:demethylmenaquinone methyltransferase



The enzyme catalyses the last step in menaquinone biosynthesis. It is able to accept substrates with varying polyprenyl side chain length (the chain length is determined by polyprenyl diphosphate synthase) [1]. The enzyme from Escherichia coli also catalyses the conversion of 2-methoxy-6-octaprenyl-1,4-benzoquinone to 5-methoxy-2-methyl-3-octaprenyl-1,4-benzoquinone during the biosynthesis of ubiquinone [4]. The enzyme probably acts on menaquinol rather than menaquinone.

Links to other databases

Enzymes and pathways: NC-IUBMB , BRENDA , ExplorEnz , ENZYME@ExPASy , KEGG , MetaCyc , UniPathway
Protein domains and families: PROSITE:PDOC00911
Structural data: CSA , EC2PDB
Gene Ontology: GO:0102094 , GO:0102955 , GO:0102027 , GO:0043770
UniProtKB/Swiss-Prot: (387) [show] [UniProt]


  1. Koike-Takeshita, A., Koyama, T., Ogura, K.
    Identification of a novel gene cluster participating in menaquinone (vitamin K2) biosynthesis. Cloning and sequence determination of the 2-heptaprenyl-1,4-naphthoquinone methyltransferase gene of Bacillus stearothermophilus.
    J. Biol. Chem. 272 : 12380-12383 (1997). [PMID: 9139683]
  2. Wissenbach, U., Ternes, D., Unden, G.
    An Escherichia coli mutant containing only demethylmenaquinone, but no menaquinone: effects on fumarate, dimethylsulfoxide, trimethylamine N-oxide and nitrate respiration.
    Arch. Microbiol. 158 : 68-73 (1992). [PMID: 1444716]
  3. Catala, F., Azerad, R., Lederer, E.
    Sur les propriétés de la desméthylménaquinone C-méthylase de Mycobacterium phlei
    Int. Z. Vitaminforsch. 40 : 363-373 (1970). [PMID: 5450997]
  4. Lee, P. T., Hsu, A. Y., Ha, H. T., Clarke, C. F.
    A C-methyltransferase involved in both ubiquinone and menaquinone biosynthesis: isolation and identification of the Escherichia coli ubiE gene.
    J. Bacteriol. 179 : 1748-1754 (1997). [PMID: 9045837]

[EC created 2009]